5o4i
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus dialyzed against solution containing 0.25 mM copper sulfate== | |
| + | <StructureSection load='5o4i' size='340' side='right' caption='[[5o4i]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5o4i]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O4I FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o4i OCA], [http://pdbe.org/5o4i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o4i RCSB], [http://www.ebi.ac.uk/pdbsum/5o4i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o4i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Laccase belongs to the family of copper-containing oxidases. A study was made of the mechanism that sustains the incorporation of copper ions into the T2/T3 centers of recombinant two-domain laccase Streptomyces griseoflavus Ac-993. The occupancy of the T3 center by copper ions was found to increase with an increasing copper content in the culture medium and after dialysis of the protein preparation against a copper sulfate-containing buffer. The T2 center was filled only when overproducer strain cells were grown at a higher copper concentration in the medium. Two-domain laccases were assumed to possess a channel that serves to deliver copper ions to the T3 center during the formation of the three-dimensional laccase conformation and dialysis of the protein preparation. A narrower channel leads to the T2 center in two-domain laccases compared with three-domain ones, rendering the center less accessible for copper atoms. The incorporation of copper ions into the T2 center of two-domain laccases is likely to occur in the course of their biosynthesis or the formation of a functional trimer. | ||
| - | + | [Incorporation of Copper Ions into T2/T3 Centers of Two-Domain Laccases].,Gabdulkhakov AG, Kostareva OS, Kolyadenko IA, Mikhaylina AO, Trubitsina LI, Tishchenko SV Mol Biol (Mosk). 2018 Jan-Feb;52(1):29-35. doi: 10.7868/S0026898418010056. PMID:29512633<ref>PMID:29512633</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Tishchenko, T | + | <div class="pdbe-citations 5o4i" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Laccase]] | ||
| + | [[Category: Gabdulkhakov, A G]] | ||
| + | [[Category: Tishchenko, T V]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Streptomyces griseoflavus]] | ||
| + | [[Category: Two-domain laccase]] | ||
Revision as of 06:29, 28 March 2018
Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus dialyzed against solution containing 0.25 mM copper sulfate
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