5o62
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of the thermobifida fusca guanidine III riboswitch with 1-Ethylguanidine.== |
| + | <StructureSection load='5o62' size='340' side='right' caption='[[5o62]], [[Resolution|resolution]] 2.12Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5o62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O62 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EGD:N-ETHYLGUANIDINE'>EGD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBV:5-BROMOCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>CBV</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o62 OCA], [http://pdbe.org/5o62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o62 RCSB], [http://www.ebi.ac.uk/pdbsum/5o62 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o62 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Riboswitches are structural elements found in mRNA molecules that couple small-molecule binding to regulation of gene expression, usually by controlling transcription or translation. We have determined high-resolution crystal structures of the ykkC guanidine III riboswitch from Thermobifida fusca. The riboswitch forms a classic H-type pseudoknot that includes a triple helix that is continuous with a central core of conserved nucleotides. These form a left-handed helical ramp of inter-nucleotide interactions, generating the guanidinium cation binding site. The ligand is hydrogen bonded to the Hoogsteen edges of two guanine bases. The binding pocket has a side opening that can accommodate a small side chain, shown by structures with bound methylguanidine, aminoguanidine, ethylguanidine, and agmatine. Comparison of the new structure with those of the guanidine I and II riboswitches reveals that evolution generated three different structural solutions for guanidine binding and subsequent gene regulation, although with some common elements. | ||
| - | + | Structure of the Guanidine III Riboswitch.,Huang L, Wang J, Wilson TJ, Lilley DMJ Cell Chem Biol. 2017 Sep 28. pii: S2451-9456(17)30319-7. doi:, 10.1016/j.chembiol.2017.08.021. PMID:28988949<ref>PMID:28988949</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5o62" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Huang, L]] | ||
| + | [[Category: Lilley, D M.J]] | ||
| + | [[Category: Wang, J]] | ||
| + | [[Category: Gene regulation]] | ||
| + | [[Category: Guanidine iii riboswitch]] | ||
| + | [[Category: Pseudoknot]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Stem-loop]] | ||
Revision as of 06:54, 18 October 2017
The structure of the thermobifida fusca guanidine III riboswitch with 1-Ethylguanidine.
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