1x8w
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1x8w.gif|left|200px]] | [[Image:1x8w.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1x8w", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1x8w| PDB=1x8w | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site''' | '''Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8W OCA]. | |
==Reference== | ==Reference== | ||
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15525509 15525509] | Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15525509 15525509] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Cech, T R.]] | [[Category: Cech, T R.]] | ||
[[Category: Gooding, A R.]] | [[Category: Gooding, A R.]] | ||
[[Category: Guo, F.]] | [[Category: Guo, F.]] | ||
| - | [[Category: | + | [[Category: Active site]] |
| - | [[Category: | + | [[Category: Base triple]] |
| - | [[Category: | + | [[Category: Catalytic mechanism]] |
| - | [[Category: | + | [[Category: Catalytic rna]] |
| - | [[Category: | + | [[Category: Conformational change]] |
| - | [[Category: | + | [[Category: Crystal structure]] |
| - | [[Category: | + | [[Category: Group i intron]] |
| - | [[Category: | + | [[Category: Guanosine binding site]] |
| - | [[Category: | + | [[Category: Metal ion]] |
| - | [[Category: | + | [[Category: Ribozyme]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:43:03 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:43, 3 May 2008
Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site
Overview
The Tetrahymena intron is an RNA catalyst, or ribozyme. As part of its self-splicing reaction, this ribozyme catalyzes phosphoryl transfer between guanosine and a substrate RNA strand. Here we report the refined crystal structure of an active Tetrahymena ribozyme in the absence of its RNA substrate at 3.8 A resolution. The 3'-terminal guanosine (omegaG), which serves as the attacking group for RNA cleavage, forms a coplanar base triple with the G264-C311 base pair, and this base triple is sandwiched by three other base triples. In addition, a metal ion is present in the active site, contacting or positioned close to the ribose of the omegaG and five phosphates. All of these phosphates have been shown to be important for catalysis. Therefore, we provide a picture of how the ribozyme active site positions both a catalytic metal ion and the nucleophilic guanosine for catalysis prior to binding its RNA substrate.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:15525509 Page seeded by OCA on Sat May 3 14:43:03 2008
