5or5

From Proteopedia

(Difference between revisions)

Revision as of 05:25, 27 June 2018

NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA

Structural highlights

5or5 is a 2 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	4lup, 2mao, 2map
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPOE_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment. The 90 member regulon consists of the genes necessary for the synthesis and maintenance of both proteins and LPS of the outer membrane.^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

Publication Abstract from PubMed

Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli sigmaE, which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors.

Structural basis for -10 promoter element melting by environmentally induced sigma factors.,Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH Nat Struct Mol Biol. 2014 Feb 16. doi: 10.1038/nsmb.2777. PMID:24531660^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Raina S, Missiakas D, Georgopoulos C. The rpoE gene encoding the sigma E (sigma 24) heat shock sigma factor of Escherichia coli. EMBO J. 1995 Mar 1;14(5):1043-55. PMID:7889935
↑ Rouviere PE, De Las Penas A, Mecsas J, Lu CZ, Rudd KE, Gross CA. rpoE, the gene encoding the second heat-shock sigma factor, sigma E, in Escherichia coli. EMBO J. 1995 Mar 1;14(5):1032-42. PMID:7889934
↑ Erickson JW, Gross CA. Identification of the sigma E subunit of Escherichia coli RNA polymerase: a second alternate sigma factor involved in high-temperature gene expression. Genes Dev. 1989 Sep;3(9):1462-71. PMID:2691330
↑ Missiakas D, Mayer MP, Lemaire M, Georgopoulos C, Raina S. Modulation of the Escherichia coli sigmaE (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins. Mol Microbiol. 1997 Apr;24(2):355-71. PMID:9159522
↑ De Las Penas A, Connolly L, Gross CA. The sigmaE-mediated response to extracytoplasmic stress in Escherichia coli is transduced by RseA and RseB, two negative regulators of sigmaE. Mol Microbiol. 1997 Apr;24(2):373-85. PMID:9159523
↑ Rhodius VA, Suh WC, Nonaka G, West J, Gross CA. Conserved and variable functions of the sigmaE stress response in related genomes. PLoS Biol. 2006 Jan;4(1):e2. PMID:16336047 doi:http://dx.doi.org/10.1371/journal.pbio.0040002
↑ Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH. Structural basis for -10 promoter element melting by environmentally induced sigma factors. Nat Struct Mol Biol. 2014 Feb 16. doi: 10.1038/nsmb.2777. PMID:24531660 doi:http://dx.doi.org/10.1038/nsmb.2777

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5or5"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5or5 is ON HOLD  until Paper Publication
+==NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA==
+<StructureSection load='5or5' size='340' side='right' caption='[[5or5]], [[NMR_Ensembles_of_Models | 13 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5or5]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OR5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OR5 FirstGlance]. <br>
+</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNG:2-DEOXY-GUANOSINE'>GNG</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lup|4lup]], [[2mao|2mao]], [[2map|2map]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5or5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5or5 OCA], [http://pdbe.org/5or5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5or5 RCSB], [http://www.ebi.ac.uk/pdbsum/5or5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5or5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/RPOE_ECOLI RPOE_ECOLI]] Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment. The 90 member regulon consists of the genes necessary for the synthesis and maintenance of both proteins and LPS of the outer membrane.<ref>PMID:7889935</ref> <ref>PMID:7889934</ref> <ref>PMID:2691330</ref> <ref>PMID:9159522</ref> <ref>PMID:9159523</ref> <ref>PMID:16336047</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli sigmaE, which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors.
-Authors: Campagne, S., Vorholt, J.A., Allain, F.H.
+Structural basis for -10 promoter element melting by environmentally induced sigma factors.,Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH Nat Struct Mol Biol. 2014 Feb 16. doi: 10.1038/nsmb.2777. PMID:24531660<ref>PMID:24531660</ref>
-Description: NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Vorholt, J.A]]
+<div class="pdbe-citations 5or5" style="background-color:#fffaf0;"></div>
-[[Category: Allain, F.H]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Allain, F H]]
 [[Category: Campagne, S]]
+[[Category: Vorholt, J A]]
+[[Category: 10 element recognition]]
+[[Category: Ecf sigma factor]]
+[[Category: Promoter melting]]
+[[Category: Transcription]]

5or5

From Proteopedia

Revision as of 05:25, 27 June 2018

NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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