1xcp

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[[Image:1xcp.gif|left|200px]]
[[Image:1xcp.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1xcp |SIZE=350|CAPTION= <scene name='initialview01'>1xcp</scene>, resolution 3.2&Aring;
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The line below this paragraph, containing "STRUCTURE_1xcp", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1xcp| PDB=1xcp | SCENE= }}
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|RELATEDENTRY=[[1de0|1de0]], [[1fp6|1FP6]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcp OCA], [http://www.ebi.ac.uk/pdbsum/1xcp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xcp RCSB]</span>
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}}
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'''Crystal Structure of the Nitrogenase Fe protein Phe135Trp with MgADP bound'''
'''Crystal Structure of the Nitrogenase Fe protein Phe135Trp with MgADP bound'''
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[[Category: Jang, S B.]]
[[Category: Jang, S B.]]
[[Category: Jeong, M S.]]
[[Category: Jeong, M S.]]
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[[Category: f135w]]
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[[Category: F135w]]
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[[Category: fe protein]]
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[[Category: Fe protein]]
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[[Category: mgadp]]
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[[Category: Mgadp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:51:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:47:46 2008''
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Revision as of 11:51, 3 May 2008

Image:1xcp.gif

Template:STRUCTURE 1xcp

Crystal Structure of the Nitrogenase Fe protein Phe135Trp with MgADP bound


Overview

The crystal structure of the Azotobacter vinelandii nitrogenase Fe protein with phenylalanine at position 135 substituted by tryptophan has been determined in MgADP-bound form by X-ray diffraction methods. Amino acid substitution studies have suggested that the phenylalanine at position 135 located near the [4Fe-4S] cluster contributes to both the midpoint potential and nucleotide-induced changes of the [4Fe-4S] cluster. Substitution of tryptophan for phenylalanine at position 135 resulted in a significant positive shift in the midpoint potential in both the isolated and nucleotide-bound states. The factors thought to control the midpoint potential of the [FeS] cluster include solvent accessibility, dipolar environment, and structural strain. The structure derived in the present work provides an explanation for the more positive midpoint potential observed in the nucleotide-bound state, and suggests important insights into the contributions of the nucleotide interaction to the conformational states that are the keys to nitrogenase catalysis. The presence of MgADP in Phe135Trp Fe protein reveals the mechanism of the long-range communication from the nucleotide-binding site that controls its affinity for the MoFe protein component.

About this Structure

1XCP is a Single protein structure of sequence from Azotobacter vinelandii. Full crystallographic information is available from OCA.

Reference

Structural basis for the changes in redox potential in the nitrogenase Phe135Trp Fe protein with MgADP Bound., Jeong MS, Jang SB, Mol Cells. 2004 Dec 31;18(3):374-82. PMID:15650336 Page seeded by OCA on Sat May 3 14:51:38 2008

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