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5ve8
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H3 1-28== | |
| + | <StructureSection load='5ve8' size='340' side='right' caption='[[5ve8]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ve8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VE8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vch|5vch]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ve8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ve8 OCA], [http://pdbe.org/5ve8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ve8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ve8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ve8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocation. Here, we report crystal structures of full-length Kluyveromyces lactis Kap123 alone and in complex with H3- and H4-NLSs. Structures reveal the unique feature of Kap123 that possesses two discrete lysine-binding pockets for NLS recognition. Structural comparison illustrates that H3- and H4-NLSs share at least one of two lysine-binding pockets, suggesting that H3- and H4-NLSs are mutually exclusive. Additionally, acetylation of key lysine residues at NLS, particularly H4-NLS diacetylation, weakens the interaction with Kap123. These data support that cytoplasmic histone H4 diacetylation weakens the Kap123-H4-NLS interaction thereby facilitating histone Kap123-H3-dependent H3:H4/Asf1 complex nuclear translocation. | ||
| - | + | Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123.,An S, Yoon J, Kim H, Song JJ, Cho US Elife. 2017 Oct 16;6. pii: e30244. doi: 10.7554/eLife.30244. PMID:29035199<ref>PMID:29035199</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 5ve8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: An, S]] | [[Category: An, S]] | ||
| - | [[Category: | + | [[Category: Cho, U S]] |
| - | [[Category: | + | [[Category: Song, J J]] |
[[Category: Yoon, J]] | [[Category: Yoon, J]] | ||
| + | [[Category: 23 heat repeats with a right-handed superhelical solenoid structure]] | ||
| + | [[Category: Bidding yeast karyopherin]] | ||
| + | [[Category: Histone nls recognition]] | ||
| + | [[Category: Protein transport]] | ||
| + | [[Category: The extra-long helix of the repeat 23]] | ||
Revision as of 06:00, 1 November 2017
Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H3 1-28
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