1xi2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1xi2.jpg|left|200px]]
[[Image:1xi2.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1xi2 |SIZE=350|CAPTION= <scene name='initialview01'>1xi2</scene>, resolution 1.5&Aring;
+
The line below this paragraph, containing "STRUCTURE_1xi2", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=CB1:5-(AZIRIDIN-1-YL)-2,4-DINITROBENZAMIDE'>CB1</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= NQO2, NMOR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1xi2| PDB=1xi2 | SCENE= }}
-
|RELATEDENTRY=[[1qr2|1QR2]], [[2qr2|2QR2]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xi2 OCA], [http://www.ebi.ac.uk/pdbsum/1xi2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xi2 RCSB]</span>
+
-
}}
+
'''Quinone Reductase 2 in Complex with Cancer Prodrug CB1954'''
'''Quinone Reductase 2 in Complex with Cancer Prodrug CB1954'''
Line 28: Line 25:
[[Category: Fu, Y.]]
[[Category: Fu, Y.]]
[[Category: Zhang, Z.]]
[[Category: Zhang, Z.]]
-
[[Category: cb1954]]
+
[[Category: Cb1954]]
-
[[Category: qr2]]
+
[[Category: Qr2]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:03:38 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:49:53 2008''
+

Revision as of 12:03, 3 May 2008

Image:1xi2.jpg

Template:STRUCTURE 1xi2

Quinone Reductase 2 in Complex with Cancer Prodrug CB1954


Overview

CB1954 is a cancer pro-drug that can be activated through reduction by Escherichia coli nitro-reductases and quinone reductases. Human quinone reductase 2 is very efficient in the activation of CB1954, approximately 3000 times more efficient than human QR1 in terms of k(cat)/K(m). We have solved the three-dimensional structure of QR2 in complex with CB1954 to a nominal resolution of 1.5A. The complex structure indicates the essentiality of the two nitro groups: one nitro group forms hydrogen bonds with the side-chain of Asn161 of QR2 to hold the other nitro group in position for the reduction. We further conclude that residue 161, an Asn in QR2 and a His in QR1, is critical in differentiating the substrate specificities of these two enzymes. Mutation of Asn161 to His161 in QR2 resulted in the total loss of the enzymatic activity towards activation of CB1954, whereas the rates of reduction towards menadione are not altered.

About this Structure

1XI2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of quinone reductase 2 in complex with cancer prodrug CB1954., Fu Y, Buryanovskyy L, Zhang Z, Biochem Biophys Res Commun. 2005 Oct 14;336(1):332-8. PMID:16129418 Page seeded by OCA on Sat May 3 15:03:38 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xi2"
Personal tools