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5xxn
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose== | |
| + | <StructureSection load='5xxn' size='340' side='right' caption='[[5xxn]], [[Resolution|resolution]] 2.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5xxn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XXN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XXN FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xxn OCA], [http://pdbe.org/5xxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xxn RCSB], [http://www.ebi.ac.uk/pdbsum/5xxn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xxn ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | BT_3567 protein, a putative beta-glucosidase from Bacteroides thetaiotaomicron, exhibits higher activity toward Sop3-5 (Sopn , n: degree of polymerization of beta-1,2-glucooligosaccharides) than toward Sop2 , unlike a known beta-glucosidase from Listeria innocua which predominantly prefers Sop2 . In the complex structure determined by soaking of a D286N mutant crystal with Sop4 , a Sop3 moiety was observed at subsites -1 to +2. The glucose moiety at subsite +2 forms a hydrogen bond with Asn81, which is replaced with Gly in the L. innocua beta-glucosidase. The Km values of the N81G mutant for Sop3-5 are much higher than those of the wild-type, suggesting that Asn81 contributes to the binding to substrates longer than Sop3 . | ||
| - | + | Function and structure relationships of a beta-1,2-glucooligosaccharide-degrading beta-glucosidase.,Ishiguro R, Tanaka N, Abe K, Nakajima M, Maeda T, Miyanaga A, Takahashi Y, Sugimoto N, Nakai H, Taguchi H FEBS Lett. 2017 Dec;591(23):3926-3936. doi: 10.1002/1873-3468.12911. Epub 2017, Nov 24. PMID:29131329<ref>PMID:29131329</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5xxn" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Abe, K]] | ||
| + | [[Category: Ishiguro, R]] | ||
| + | [[Category: Maeda, T]] | ||
| + | [[Category: Miyanaga, A]] | ||
[[Category: Nakai, H]] | [[Category: Nakai, H]] | ||
[[Category: Nakajima, M]] | [[Category: Nakajima, M]] | ||
[[Category: Sugimono, N]] | [[Category: Sugimono, N]] | ||
[[Category: Taguchi, H]] | [[Category: Taguchi, H]] | ||
| - | [[Category: Ishiguro, R]] | ||
[[Category: Takahashi, Y]] | [[Category: Takahashi, Y]] | ||
[[Category: Tanaka, N]] | [[Category: Tanaka, N]] | ||
| - | [[Category: | + | [[Category: Beta-glucosidase]] |
| - | [[Category: | + | [[Category: Glycoside hydrolase family 3]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
Revision as of 07:01, 13 December 2017
Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose
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