1xsk

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[[Image:1xsk.gif|left|200px]]
[[Image:1xsk.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1xsk |SIZE=350|CAPTION= <scene name='initialview01'>1xsk</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_1xsk", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYF:5(R)-5-FLUORO-BETA-D-XYLOPYRANOSYL-ENZYME+INTERMEDIATE'>XYF</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= yicI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1xsk| PDB=1xsk | SCENE= }}
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|RELATEDENTRY=[[1xsi|1XSI]], [[1xsj|1XSJ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsk OCA], [http://www.ebi.ac.uk/pdbsum/1xsk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xsk RCSB]</span>
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}}
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'''Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate'''
'''Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate'''
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[[Category: Strynadka, N C.]]
[[Category: Strynadka, N C.]]
[[Category: Withers, S G.]]
[[Category: Withers, S G.]]
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[[Category: beta(8)alpha(8) barrel and beta sandwich domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:27:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:54:05 2008''
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Revision as of 12:27, 3 May 2008

Image:1xsk.gif

Template:STRUCTURE 1xsk

Structure of a Family 31 alpha glycosidase glycosyl-enzyme intermediate


Overview

We have determined the first structure of a family 31 alpha-glycosidase, that of YicI from Escherichia coli, both free and trapped as a 5-fluoroxylopyranosyl-enzyme intermediate via reaction with 5-fluoro-alpha-D-xylopyranosyl fluoride. Our 2.2-A resolution structure shows an intimately associated hexamer with structural elements from several monomers converging at each of the six active sites. Our kinetic and mass spectrometry analyses verified several of the features observed in our structural data, including a covalent linkage from the carboxylate side chain of the identified nucleophile Asp(416) to C-1 of the sugar ring. Structure-based sequence comparison of YicI with the mammalian alpha-glucosidases lysosomal alpha-glucosidase and sucrase-isomaltase predicts a high level of structural similarity and provides a foundation for understanding the various mutations of these enzymes that elicit human disease.

About this Structure

1XSK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate., Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC, J Biol Chem. 2005 Jan 21;280(3):2105-15. Epub 2004 Oct 22. PMID:15501829 Page seeded by OCA on Sat May 3 15:27:34 2008

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