1xss
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1xss.gif|left|200px]] | [[Image:1xss.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1xss", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1xss| PDB=1xss | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.''' | '''Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. | |
==Reference== | ==Reference== | ||
Semi-rational engineering of a coral fluorescent protein into an efficient highlighter., Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A, EMBO Rep. 2005 Mar;6(3):233-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15731765 15731765] | Semi-rational engineering of a coral fluorescent protein into an efficient highlighter., Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A, EMBO Rep. 2005 Mar;6(3):233-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15731765 15731765] | ||
| - | [[Category: Favia favus]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Karasawa, S.]] | [[Category: Karasawa, S.]] | ||
[[Category: Miyawaki, A.]] | [[Category: Miyawaki, A.]] | ||
| Line 30: | Line 25: | ||
[[Category: Shimizu, H.]] | [[Category: Shimizu, H.]] | ||
[[Category: Tsutsui, H.]] | [[Category: Tsutsui, H.]] | ||
| - | [[Category: | + | [[Category: Fluorescent protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:28:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:28, 3 May 2008
Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.
Overview
Kaede is a natural photoconvertible fluorescent protein found in the coral Trachyphyllia geoffroyi. It contains a tripeptide, His 62-Tyr 63-Gly 64, which acts as a green chromophore that is photoconvertible to red following (ultra-) violet irradiation. Here, we report the molecular cloning and crystal structure determination of a new fluorescent protein, KikG, from the coral Favia favus, and its in vitro evolution conferring green-to-red photoconvertibility. Substitution of the His 62-Tyr 63-Gly 64 sequence into the native protein provided only negligible photoconversion. On the basis of the crystal structure, semi-rational mutagenesis of the amino acids surrounding the chromophore was performed, leading to the generation of an efficient highlighter, KikGR. Within mammalian cells, KikGR is more efficiently photoconverted and is several-fold brighter in both the green and red states than Kaede. In addition, KikGR was successfully photoconverted using two-photon excitation microscopy at 760 nm, ensuring optical cell labelling with better spatial discrimination in thick and highly scattering tissues.
About this Structure
Full crystallographic information is available from OCA.
Reference
Semi-rational engineering of a coral fluorescent protein into an efficient highlighter., Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A, EMBO Rep. 2005 Mar;6(3):233-8. PMID:15731765 Page seeded by OCA on Sat May 3 15:28:02 2008
