1xua

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[[Image:1xua.jpg|left|200px]]
[[Image:1xua.jpg|left|200px]]
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{{Structure
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|PDB= 1xua |SIZE=350|CAPTION= <scene name='initialview01'>1xua</scene>, resolution 1.90&Aring;
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|LIGAND= <scene name='pdbligand=HHA:(2S,3S)-TRANS-2,3-DIHYDRO-3-HYDROXYANTHRANILIC+ACID'>HHA</scene>
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{{STRUCTURE_1xua| PDB=1xua | SCENE= }}
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|RELATEDENTRY=[[1u1v|1U1V]], [[1u1w|1U1W]], [[1u1x|1U1X]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xua OCA], [http://www.ebi.ac.uk/pdbsum/1xua PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xua RCSB]</span>
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'''Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens'''
'''Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens'''
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[[Category: Thomashow, L S.]]
[[Category: Thomashow, L S.]]
[[Category: Tong, L.]]
[[Category: Tong, L.]]
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[[Category: phenazine biosynthesis]]
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[[Category: Phenazine biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:31:03 2008''
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Revision as of 12:31, 3 May 2008

Template:STRUCTURE 1xua

Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens


Overview

Phenazines produced by Pseudomonas and Streptomyces spp. are heterocyclic nitrogen-containing metabolites with antibiotic, antitumor, and antiparasitic activity. The antibiotic properties of pyocyanin, produced by Pseudomonas aeruginosa, were recognized in the 1890s, although this blue phenazine is now known to be a virulence factor in human disease. Despite their biological significance, the biosynthesis of phenazines is not fully understood. Here we present structural and functional studies of PhzF, an enzyme essential for phenazine synthesis in Pseudomonas spp. PhzF shares topology with diaminopimelate epimerase DapF but lacks the same catalytic residues. The structure of PhzF in complex with its substrate, trans-2,3-dihydro-3-hydroxyanthranilic acid, suggests that it is an isomerase using the conserved glutamate E45 to abstract a proton from C3 of the substrate. The proton is returned to C1 of the substrate after rearrangement of the double-bond system, yielding an enol that converts to the corresponding ketone. PhzF is a dimer that may be bifunctional, providing a shielded cavity for ketone dimerization via double Schiff-base formation to produce the phenazine scaffold. Our proposed mechanism is supported by mass and NMR spectroscopy. The results are discussed in the context of related structures and protein sequences of unknown biochemical function.

About this Structure

1XUA is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

Reference

Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens., Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV, Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16431-6. Epub 2004 Nov 15. PMID:15545603 Page seeded by OCA on Sat May 3 15:31:03 2008

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