1y3h

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[[Image:1y3h.gif|left|200px]]
[[Image:1y3h.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1y3h |SIZE=350|CAPTION= <scene name='initialview01'>1y3h</scene>, resolution 2.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1y3h", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_kinase NAD(+) kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.23 2.7.1.23] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= Ppnk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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|DOMAIN=
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{{STRUCTURE_1y3h| PDB=1y3h | SCENE= }}
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|RELATEDENTRY=[[1y3i|1Y3I]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3h OCA], [http://www.ebi.ac.uk/pdbsum/1y3h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y3h RCSB]</span>
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}}
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'''Crystal Structure of Inorganic Polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis'''
'''Crystal Structure of Inorganic Polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis'''
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NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex., Mori S, Yamasaki M, Maruyama Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K, Biochem Biophys Res Commun. 2005 Feb 11;327(2):500-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15629142 15629142]
NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex., Mori S, Yamasaki M, Maruyama Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K, Biochem Biophys Res Commun. 2005 Feb 11;327(2):500-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15629142 15629142]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: NAD(+) kinase]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Hashimoto, W.]]
[[Category: Hashimoto, W.]]
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[[Category: Murata, K.]]
[[Category: Murata, K.]]
[[Category: Yamasaki, M.]]
[[Category: Yamasaki, M.]]
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[[Category: kawai, S.]]
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[[Category: Kawai, S.]]
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[[Category: atp]]
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[[Category: Atp]]
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[[Category: nad]]
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[[Category: Nad]]
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[[Category: nad kinase]]
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[[Category: Nad kinase]]
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[[Category: polyphosphate]]
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[[Category: Polyphosphate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:50:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:58:19 2008''
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Revision as of 12:50, 3 May 2008

Image:1y3h.gif

Template:STRUCTURE 1y3h

Crystal Structure of Inorganic Polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis


Overview

NAD kinase is a key enzyme in NADP biosynthesis. We solved the crystal structure of polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis (Ppnk) complexed with NAD (Ppnk-NAD) at 2.6A resolution using apo-Ppnk structure solved in this work, and revealed the details of the structure and NAD-binding site. Superimposition of tertiary structures of apo-Ppnk and Ppnk-NAD demonstrated a substantial conformational difference in a loop (Ppnk-flexible loop). As a quaternary structure, these Ppnk structures exhibited tetramer as in solution condition. Notably, the Ppnk-flexible loop was involved in the intersubunit contact and probably related to the NAD-binding of the other subunit. Furthermore, the two residues (Asp189, His226) substantially contributed to creating NAD-binding site on the other subunit. The two residues and the residues involved in NAD-binding were conserved. However, residues corresponding to the Ppnk-flexible loop were not conserved, making us to speculate that the Ppnk-flexible loop may be Ppnk-specific.

About this Structure

1Y3H is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex., Mori S, Yamasaki M, Maruyama Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K, Biochem Biophys Res Commun. 2005 Feb 11;327(2):500-8. PMID:15629142 Page seeded by OCA on Sat May 3 15:50:21 2008

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