This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1y3h

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1y3h.gif|left|200px]]
[[Image:1y3h.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1y3h |SIZE=350|CAPTION= <scene name='initialview01'>1y3h</scene>, resolution 2.80&Aring;
+
The line below this paragraph, containing "STRUCTURE_1y3h", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_kinase NAD(+) kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.23 2.7.1.23] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= Ppnk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1y3h| PDB=1y3h | SCENE= }}
-
|RELATEDENTRY=[[1y3i|1Y3I]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y3h OCA], [http://www.ebi.ac.uk/pdbsum/1y3h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y3h RCSB]</span>
+
-
}}
+
'''Crystal Structure of Inorganic Polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis'''
'''Crystal Structure of Inorganic Polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis'''
Line 24: Line 21:
NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex., Mori S, Yamasaki M, Maruyama Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K, Biochem Biophys Res Commun. 2005 Feb 11;327(2):500-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15629142 15629142]
NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex., Mori S, Yamasaki M, Maruyama Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K, Biochem Biophys Res Commun. 2005 Feb 11;327(2):500-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15629142 15629142]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
-
[[Category: NAD(+) kinase]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Hashimoto, W.]]
[[Category: Hashimoto, W.]]
Line 33: Line 29:
[[Category: Murata, K.]]
[[Category: Murata, K.]]
[[Category: Yamasaki, M.]]
[[Category: Yamasaki, M.]]
-
[[Category: kawai, S.]]
+
[[Category: Kawai, S.]]
-
[[Category: atp]]
+
[[Category: Atp]]
-
[[Category: nad]]
+
[[Category: Nad]]
-
[[Category: nad kinase]]
+
[[Category: Nad kinase]]
-
[[Category: polyphosphate]]
+
[[Category: Polyphosphate]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:50:21 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:58:19 2008''
+

Revision as of 12:50, 3 May 2008

Image:1y3h.gif

Template:STRUCTURE 1y3h

Crystal Structure of Inorganic Polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis


Overview

NAD kinase is a key enzyme in NADP biosynthesis. We solved the crystal structure of polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis (Ppnk) complexed with NAD (Ppnk-NAD) at 2.6A resolution using apo-Ppnk structure solved in this work, and revealed the details of the structure and NAD-binding site. Superimposition of tertiary structures of apo-Ppnk and Ppnk-NAD demonstrated a substantial conformational difference in a loop (Ppnk-flexible loop). As a quaternary structure, these Ppnk structures exhibited tetramer as in solution condition. Notably, the Ppnk-flexible loop was involved in the intersubunit contact and probably related to the NAD-binding of the other subunit. Furthermore, the two residues (Asp189, His226) substantially contributed to creating NAD-binding site on the other subunit. The two residues and the residues involved in NAD-binding were conserved. However, residues corresponding to the Ppnk-flexible loop were not conserved, making us to speculate that the Ppnk-flexible loop may be Ppnk-specific.

About this Structure

1Y3H is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex., Mori S, Yamasaki M, Maruyama Y, Momma K, Kawai S, Hashimoto W, Mikami B, Murata K, Biochem Biophys Res Commun. 2005 Feb 11;327(2):500-8. PMID:15629142 Page seeded by OCA on Sat May 3 15:50:21 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1y3h"
Personal tools