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1y4w

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[[Image:1y4w.jpg|left|200px]]
[[Image:1y4w.jpg|left|200px]]
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{{Structure
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|PDB= 1y4w |SIZE=350|CAPTION= <scene name='initialview01'>1y4w</scene>, resolution 1.55&Aring;
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The line below this paragraph, containing "STRUCTURE_1y4w", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] </span>
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{{STRUCTURE_1y4w| PDB=1y4w | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4w OCA], [http://www.ebi.ac.uk/pdbsum/1y4w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y4w RCSB]</span>
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'''Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21'''
'''Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21'''
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[[Category: Polikarpov, I.]]
[[Category: Polikarpov, I.]]
[[Category: Rojas, A L.]]
[[Category: Rojas, A L.]]
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[[Category: aspergillus awamori]]
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[[Category: Aspergillus awamori]]
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[[Category: crystallographic structure]]
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[[Category: Crystallographic structure]]
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[[Category: exo-inulinase]]
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[[Category: Exo-inulinase]]
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[[Category: glycoside hydrolase family 32]]
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[[Category: Glycoside hydrolase family 32]]
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[[Category: native structure]]
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[[Category: Native structure]]
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[[Category: x-ray structure]]
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[[Category: X-ray structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:52:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:58:51 2008''
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Revision as of 12:52, 3 May 2008

Image:1y4w.jpg

Template:STRUCTURE 1y4w

Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21


Overview

Exo-inulinases hydrolyze terminal, non-reducing 2,1-linked and 2,6-linked beta-d-fructofuranose residues in inulin, levan and sucrose releasing beta-d-fructose. We present the X-ray structure at 1.55A resolution of exo-inulinase from Aspergillus awamori, a member of glycoside hydrolase family 32, solved by single isomorphous replacement with the anomalous scattering method using the heavy-atom sites derived from a quick cryo-soaking technique. The tertiary structure of this enzyme folds into two domains: the N-terminal catalytic domain of an unusual five-bladed beta-propeller fold and the C-terminal domain folded into a beta-sandwich-like structure. Its structural architecture is very similar to that of another member of glycoside hydrolase family 32, invertase (beta-fructosidase) from Thermotoga maritima, determined recently by X-ray crystallography The exo-inulinase is a glycoprotein containing five N-linked oligosaccharides. Two crystal forms obtained under similar crystallization conditions differ by the degree of protein glycosylation. The X-ray structure of the enzyme:fructose complex, at a resolution of 1.87A, reveals two catalytically important residues: Asp41 and Glu241, a nucleophile and a catalytic acid/base, respectively. The distance between the side-chains of these residues is consistent with a double displacement mechanism of reaction. Asp189, which is part of the Arg-Asp-Pro motif, provides hydrogen bonds important for substrate recognition.

About this Structure

1Y4W is a Single protein structure of sequence from Aspergillus awamori. Full crystallographic information is available from OCA.

Reference

Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition., Nagem RA, Rojas AL, Golubev AM, Korneeva OS, Eneyskaya EV, Kulminskaya AA, Neustroev KN, Polikarpov I, J Mol Biol. 2004 Nov 19;344(2):471-80. PMID:15522299 Page seeded by OCA on Sat May 3 15:52:54 2008

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