5vy1

Structural highlights

Function

[TDRKH_BOMMO] Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity (PubMed:23970546, PubMed:26919431). The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins (Siwi or Ago3) and govern the methylation and subsequent repression of transposons (PubMed:23970546, PubMed:26919431). Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs: acts by recruiting the exonuclease PNLDC1/trimmer to Siwi-bound pre-piRNAs (PubMed:26919431).^{[1] [2]}

References

1. ↑ Honda S, Kirino Y, Maragkakis M, Alexiou P, Ohtaki A, Murali R, Mourelatos Z, Kirino Y. Mitochondrial protein BmPAPI modulates the length of mature piRNAs. RNA. 2013 Oct;19(10):1405-18. doi: 10.1261/rna.040428.113. Epub 2013 Aug 22. PMID:23970546 doi:http://dx.doi.org/10.1261/rna.040428.113
2. ↑ Izumi N, Shoji K, Sakaguchi Y, Honda S, Kirino Y, Suzuki T, Katsuma S, Tomari Y. Identification and Functional Analysis of the Pre-piRNA 3' Trimmer in Silkworms. Cell. 2016 Feb 25;164(5):962-73. doi: 10.1016/j.cell.2016.01.008. PMID:26919431 doi:http://dx.doi.org/10.1016/j.cell.2016.01.008