1yba
From Proteopedia
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'''The active form of phosphoglycerate dehydrogenase''' | '''The active form of phosphoglycerate dehydrogenase''' | ||
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[[Category: Banaszak, L J.]] | [[Category: Banaszak, L J.]] | ||
[[Category: Thompson, J R.]] | [[Category: Thompson, J R.]] | ||
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Revision as of 13:06, 3 May 2008
The active form of phosphoglycerate dehydrogenase
Overview
An active conformation of phosphoglycerate dehydrogenase (PGDH) from Escherichia coli has been obtained using X-ray crystallography. The X-ray crystal structure is used to examine the potential intermediates for V(max) regulation, for the redox reaction, and for cooperative effects of serine binding. The crystal structure at 2.2 A resolution contains bound NAD(+) cofactor, either sulfate or phosphate anions, and alpha-ketoglutarate, a nonphysiological substrate. A PGDH subunit is formed from three distinct domains: regulatory (RBD), substrate (SBD), and nucleotide binding (NBD). The crystal conformation of the homotetramer points to the fact that, in the absence of serine, coordinated movement of the RBD-SBD domains occurs relative to the NBD. The result is a conformational change involving the steric relationships of both the domains and the subunits. Within the active site of each subunit is a bound molecule of alpha-ketoglutarate and the coenzyme, NAD. The catalytic or active site cleft is changed slightly although it is still solvent exposed; therefore, the catalytic reaction probably involves additional conformational changes. By comparing the inhibited with the uninhibited complex, it is possible to describe changes in conformation that are involved in the inhibitory signal transduction of serine.
About this Structure
1YBA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Vmax regulation through domain and subunit changes. The active form of phosphoglycerate dehydrogenase., Thompson JR, Bell JK, Bratt J, Grant GA, Banaszak LJ, Biochemistry. 2005 Apr 19;44(15):5763-73. PMID:15823035 Page seeded by OCA on Sat May 3 16:06:27 2008
