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1ynu

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[[Image:1ynu.gif|left|200px]]
[[Image:1ynu.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ynu |SIZE=350|CAPTION= <scene name='initialview01'>1ynu</scene>, resolution 2.25&Aring;
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The line below this paragraph, containing "STRUCTURE_1ynu", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PY4:2-[O-PHOSPHONOPYRIDOXYL]-AMINO-+BUTYRIC+ACID'>PY4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/1-aminocyclopropane-1-carboxylate_synthase 1-aminocyclopropane-1-carboxylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.14 4.4.1.14] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ACS-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3750 Malus x domestica])
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|DOMAIN=
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{{STRUCTURE_1ynu| PDB=1ynu | SCENE= }}
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|RELATEDENTRY=[[1b8g|1B8G]], [[1m7y|1M7Y]], [[1m4n|1M4N]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ynu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ynu OCA], [http://www.ebi.ac.uk/pdbsum/1ynu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ynu RCSB]</span>
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}}
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'''Crystal structure of apple ACC synthase in complex with L-vinylglycine'''
'''Crystal structure of apple ACC synthase in complex with L-vinylglycine'''
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[[Category: Kirsch, J F.]]
[[Category: Kirsch, J F.]]
[[Category: Tschopp, M.]]
[[Category: Tschopp, M.]]
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[[Category: lyase]]
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[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:33:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:14:48 2008''
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Revision as of 13:33, 3 May 2008

Image:1ynu.gif

Template:STRUCTURE 1ynu

Crystal structure of apple ACC synthase in complex with L-vinylglycine


Overview

L-Vinylglycine (L-VG) is both a substrate for and a mechanism-based inhibitor of 1-aminocyclopropane-1-carboxylate (ACC) synthase. The ratio of the rate constants for catalytic conversion to alpha-ketobutyrate and ammonia to inactivation is 500/1. The crystal structure of the covalent adduct of the inactivated enzyme was determined at 2.25 Angstroms resolution. The active site contains an external aldimine of the adduct of L-VG with the pyridoxal 5'-phosphate cofactor. The side chain gamma-carbon of L-VG is covalently bound to the epsilon-amino group of Lys273. This species corresponds to one of the two alternatives proposed by Feng and Kirsch [Feng, L. and Kirsch, J.F. (2000) L-Vinylglycine is an alternative substrate as well as a mechanism-based inhibitor of 1-aminocyclopropane-1-carboxylate synthase. Biochemistry 39, 2436-2444] and presumably results from Michael addition to a vinylglycine ketimine intermediate.

About this Structure

1YNU is a Single protein structure of sequence from Malus x domestica. Full crystallographic information is available from OCA.

Reference

Structure of ACC synthase inactivated by the mechanism-based inhibitor L-vinylglycine., Capitani G, Tschopp M, Eliot AC, Kirsch JF, Grutter MG, FEBS Lett. 2005 Apr 25;579(11):2458-62. PMID:15848188 Page seeded by OCA on Sat May 3 16:33:35 2008

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