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5oqr

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Revision as of 07:30, 2 September 2020

Crystal structure of the S. pombe condensin Cnd3-Cnd2 subcomplex

Structural highlights

5oqr is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5oqn, 5oqo, 5oqp, 5oqq
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CND3_SCHPO] Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase.^[1] [CND2_SCHPO] Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex probably also plays a role during interphase in processes such as DNA repair.^[2] ^[3]

Publication Abstract from PubMed

Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin rearrangements that take place during cell divisions. Here, we identify a direct DNA-binding site in the eukaryotic condensin complex, which is formed by its Ycg1Cnd3 HEAT-repeat and Brn1Cnd2 kleisin subunits. DNA co-crystal structures reveal a conserved, positively charged groove that accommodates the DNA double helix. A peptide loop of the kleisin subunit encircles the bound DNA and, like a safety belt, prevents its dissociation. Firm closure of the kleisin loop around DNA is essential for the association of condensin complexes with chromosomes and their DNA-stimulated ATPase activity. Our data suggest a sophisticated molecular basis for anchoring condensin complexes to chromosomes that enables the formation of large-sized chromatin loops.

Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.,Kschonsak M, Merkel F, Bisht S, Metz J, Rybin V, Hassler M, Haering CH Cell. 2017 Oct 4. pii: S0092-8674(17)31057-7. doi: 10.1016/j.cell.2017.09.008. PMID:28988770^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sutani T, Yuasa T, Tomonaga T, Dohmae N, Takio K, Yanagida M. Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4. Genes Dev. 1999 Sep 1;13(17):2271-83. PMID:10485849
↑ Sutani T, Yuasa T, Tomonaga T, Dohmae N, Takio K, Yanagida M. Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4. Genes Dev. 1999 Sep 1;13(17):2271-83. PMID:10485849
↑ Aono N, Sutani T, Tomonaga T, Mochida S, Yanagida M. Cnd2 has dual roles in mitotic condensation and interphase. Nature. 2002 May 9;417(6885):197-202. PMID:12000964 doi:http://dx.doi.org/10.1038/417197a
↑ Kschonsak M, Merkel F, Bisht S, Metz J, Rybin V, Hassler M, Haering CH. Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes. Cell. 2017 Oct 4. pii: S0092-8674(17)31057-7. doi: 10.1016/j.cell.2017.09.008. PMID:28988770 doi:http://dx.doi.org/10.1016/j.cell.2017.09.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5oqr"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the S. pombe condensin Cnd3-Cnd2 subcomplex==
-<StructureSection load='5oqr' size='340' side='right' caption='[[5oqr]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
+<StructureSection load='5oqr' size='340' side='right'caption='[[5oqr]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5oqr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5oqr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OQR FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5oqn|5oqn]], [[5oqo|5oqo]], [[5oqp|5oqp]], [[5oqq|5oqq]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oqr OCA], [http://pdbe.org/5oqr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oqr RCSB], [http://www.ebi.ac.uk/pdbsum/5oqr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oqr ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5oqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oqr OCA], [http://pdbe.org/5oqr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oqr RCSB], [http://www.ebi.ac.uk/pdbsum/5oqr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oqr ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 5oqr" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Condensin|Condensin]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Haering, C H]]
 [[Category: Hassler, M]]

5oqr

From Proteopedia

Revision as of 07:30, 2 September 2020

Crystal structure of the S. pombe condensin Cnd3-Cnd2 subcomplex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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