1yvb
From Proteopedia
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'''the Plasmodium falciparum Cysteine Protease Falcipain-2''' | '''the Plasmodium falciparum Cysteine Protease Falcipain-2''' | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Wang, S X.]] | [[Category: Wang, S X.]] | ||
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Revision as of 13:50, 3 May 2008
the Plasmodium falciparum Cysteine Protease Falcipain-2
Overview
Falcipain-2 (FP2), the major cysteine protease of the human malaria parasite Plasmodium falciparum, is a hemoglobinase and promising drug target. Here we report the crystal structure of FP2 in complex with a protease inhibitor, cystatin. The FP2 structure reveals two previously undescribed cysteine protease structural motifs, designated FP2(nose) and FP2(arm), in addition to details of the active site that will help focus inhibitor design. Unlike most cysteine proteases, FP2 does not require a prodomain but only the short FP2(nose) motif to correctly fold and gain catalytic activity. Our structure and mutagenesis data suggest a molecular basis for this unique mechanism by highlighting the functional role of two Tyr within FP2(nose) and a conserved Glu outside this motif. The FP2(arm) motif is required for hemoglobinase activity. The structure reveals topographic features and a negative charge cluster surrounding FP2(arm) that suggest it may serve as an exo-site for hemoglobin binding. Motifs similar to FP2(nose) and FP2(arm) are found only in related plasmodial proteases, suggesting that they confer malaria-specific functions.
About this Structure
1YVB is a Protein complex structure of sequences from Gallus gallus and Plasmodium falciparum 3d7. Full crystallographic information is available from OCA.
Reference
Structural basis for unique mechanisms of folding and hemoglobin binding by a malarial protease., Wang SX, Pandey KC, Somoza JR, Sijwali PS, Kortemme T, Brinen LS, Fletterick RJ, Rosenthal PJ, McKerrow JH, Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11503-8. Epub 2006 Jul 24. PMID:16864794 Page seeded by OCA on Sat May 3 16:50:04 2008
