1yzc
From Proteopedia
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'''The solution structure of a redesigned apocytochrome B562 (Rd-apocyt b562) with the N- and a part of the C-terminal helices unfolded''' | '''The solution structure of a redesigned apocytochrome B562 (Rd-apocyt b562) with the N- and a part of the C-terminal helices unfolded''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZC OCA]. | |
==Reference== | ==Reference== | ||
A protein folding pathway with multiple folding intermediates at atomic resolution., Feng H, Zhou Z, Bai Y, Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5026-31. Epub 2005 Mar 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15793003 15793003] | A protein folding pathway with multiple folding intermediates at atomic resolution., Feng H, Zhou Z, Bai Y, Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5026-31. Epub 2005 Mar 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15793003 15793003] | ||
| - | [[Category: Homo sapiens]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: BSGC, Berkeley Structural Genomics Center.]] | [[Category: BSGC, Berkeley Structural Genomics Center.]] | ||
[[Category: Bai, Y.]] | [[Category: Bai, Y.]] | ||
[[Category: Feng, H.]] | [[Category: Feng, H.]] | ||
[[Category: Zhou, Z.]] | [[Category: Zhou, Z.]] | ||
| - | [[Category: | + | [[Category: Berkeley structural genomics center]] |
| - | [[Category: | + | [[Category: Bsgc]] |
| - | [[Category: | + | [[Category: Folding intermediate]] |
| - | [[Category: | + | [[Category: Native-state hydrogen exchange]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Protein engineering]] |
| - | [[Category: | + | [[Category: Protein structure]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:59:43 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:59, 3 May 2008
The solution structure of a redesigned apocytochrome B562 (Rd-apocyt b562) with the N- and a part of the C-terminal helices unfolded
Overview
Using native-state hydrogen-exchange-directed protein engineering and multidimensional NMR, we determined the high-resolution structure (rms deviation, 1.1 angstroms) for an intermediate of the four-helix bundle protein: Rd-apocytochrome b562. The intermediate has the N-terminal helix and a part of the C-terminal helix unfolded. In earlier studies, we also solved the structures of two other folding intermediates for the same protein: one with the N-terminal helix alone unfolded and the other with a reorganized hydrophobic core. Together, these structures provide a description of a protein folding pathway with multiple intermediates at atomic resolution. The two general features for the intermediates are (i) native-like backbone topology and (ii) nonnative side-chain interactions. These results have implications for important issues in protein folding studies, including large-scale conformation search, -value analysis, and computer simulations.
About this Structure
Full crystallographic information is available from OCA.
Reference
A protein folding pathway with multiple folding intermediates at atomic resolution., Feng H, Zhou Z, Bai Y, Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5026-31. Epub 2005 Mar 25. PMID:15793003 Page seeded by OCA on Sat May 3 16:59:43 2008
