1yz9
From Proteopedia
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'''Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution''' | '''Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution''' | ||
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[[Category: Tropea, J E.]] | [[Category: Tropea, J E.]] | ||
[[Category: Waugh, D S.]] | [[Category: Waugh, D S.]] | ||
| - | [[Category: | + | [[Category: Double-stranded rna]] |
| - | [[Category: | + | [[Category: Endonucleolytic cleavage]] |
| - | [[Category: | + | [[Category: Ribonuclease iii]] |
| - | [[Category: | + | [[Category: Rna interference]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:59:33 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:59, 3 May 2008
Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution
Overview
Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures, including the endonuclease domain of RNase III with and without bound metal ions and a dsRNA binding protein complexed with dsRNA. We also reported a noncatalytic assembly observed in the crystal structure of an RNase III mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We hypothesize that the RNase III*dsRNA complex can exist in two functional forms, a catalytic complex and a noncatalytic assembly, and that in between the two forms there may be intermediate states. Here, we present four crystal structures of RNase III complexed with dsRNA, representing possible intermediates.
About this Structure
1YZ9 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Intermediate states of ribonuclease III in complex with double-stranded RNA., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Structure. 2005 Oct;13(10):1435-42. PMID:16216575 Page seeded by OCA on Sat May 3 16:59:33 2008
