Monocyte chemoattractant protein
From Proteopedia
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{{#tree:id=OrganizedByTopic|openlevels=0| | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
| - | * Monocyte chemoattractant protein 1 | + | * Monocyte chemoattractant protein 1 1 or C-C motif chemokine 2 or CCL2 |
**[[1dok]], [[1dol]] – hMCP-1 (mutant) - human<br /> | **[[1dok]], [[1dol]] – hMCP-1 (mutant) - human<br /> | ||
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**[[4zk9]] – hMCP-1 + chemokine binding protein<br /> | **[[4zk9]] – hMCP-1 + chemokine binding protein<br /> | ||
**[[4r8i]] – hMCP-1 + RNA<br /> | **[[4r8i]] – hMCP-1 + RNA<br /> | ||
| + | **[[7so0]], [[8fj0]] – hMCP-1 + evasin<br /> | ||
| - | * Monocyte chemoattractant protein 2 | + | * Monocyte chemoattractant protein 2 or CCL8 or C-C motif chemokine 8 |
| + | **[[7s5a]] – hMCP-2 <br /> | ||
**[[1esr]] – hMCP-2 (mutant) <br /> | **[[1esr]] – hMCP-2 (mutant) <br /> | ||
| - | * Monocyte chemoattractant protein 3 | + | * Monocyte chemoattractant protein 3 or C-C motif chemokine 7 or CCL7 |
**[[1ncv]], [[1bo0]] – hMCP-3 - NMR<br /> | **[[1ncv]], [[1bo0]] – hMCP-3 - NMR<br /> | ||
**[[4zkc]] – hMCP-3 + chemokine binding protein<br /> | **[[4zkc]] – hMCP-3 + chemokine binding protein<br /> | ||
| + | **[[7s58]], [[7s59]], [[7scu]] – hMCP-3 + evasin<br /> | ||
| + | **[[8fk6]], [[8fk8]] – hMCP-3 + evasin<br /> | ||
| + | **[[8fj3]] – hMCP-3 (mutant) + evasin<br /> | ||
* Monocyte chemoattractant protein 4 | * Monocyte chemoattractant protein 4 | ||
Revision as of 07:16, 6 July 2023
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Synthesis
The protein human CCL2 has been synthesized using a combination of solid phase peptide synthesis (SPPS) and native chemical ligation (NCL). The thioester-peptide segment was synthesized using the sulfonamide safety-catch linker and 9-fluorenylmethoxycarbonyl (Fmoc) SPPS, and pseudoproline dipeptides were used to facilitate the synthesis of both CCL2 fragments. After assembly of the full-length peptide chain by NCL, a glutathione redox buffer was used to fold and oxidize the CCL2 protein. CCL2 was crystallized and the structure was determined by X-ray diffraction at 1.9-A resolution. This is probably one of the first crystal structures of a protein prepared using the sulfonamide safety-catch linker and NCL.
3D structures of Monocyte chemoattractant protein
Updated on 06-July-2023
References
https://fr.wikipedia.org/wiki/CCL2 http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1DOK http://www.uniprot.org/uniprot/P13500#interaction http://www.rcsb.org/pdb/explore/explore.do?structureId=3IFD
- ↑ Carr MW, Roth SJ, Luther E, Rose SS, Springer TA. Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3652-6. PMID:8170963
- ↑ Ito Y, Ishiguro H, Kobayashi N, Hasumi H, Watanabe M, Yao M, Uemura H. Adipocyte-derived monocyte chemotactic protein-1 (MCP-1) promotes prostate cancer progression through the induction of MMP-2 activity. Prostate. 2015 Jul 1;75(10):1009-19. doi: 10.1002/pros.22972. Epub 2015 Apr 27. PMID:25917126 doi:http://dx.doi.org/10.1002/pros.22972
- ↑ Lubkowski J, Bujacz G, Boque L, Domaille PJ, Handel TM, Wlodawer A. The structure of MCP-1 in two crystal forms provides a rare example of variable quaternary interactions. Nat Struct Biol. 1997 Jan;4(1):64-9. PMID:8989326
Proteopedia Page Contributors and Editors (what is this?)
Coline Perrin, Alexane Caignard, Michal Harel, Alexander Berchansky, Joel L. Sussman
