1z5u

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[[Image:1z5u.gif|left|200px]]
[[Image:1z5u.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1z5u |SIZE=350|CAPTION= <scene name='initialview01'>1z5u</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1z5u", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CMP:ADENOSINE-3&#39;,5&#39;-CYCLIC-MONOPHOSPHATE'>CMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= aphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium])
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-->
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|DOMAIN=
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{{STRUCTURE_1z5u| PDB=1z5u | SCENE= }}
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|RELATEDENTRY=[[1z5g|1Z5G]], [[1z88|1Z88]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z5u OCA], [http://www.ebi.ac.uk/pdbsum/1z5u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z5u RCSB]</span>
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}}
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'''Crystal structure of S. typhimurium AphA complexed with cyclic-AMP'''
'''Crystal structure of S. typhimurium AphA complexed with cyclic-AMP'''
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[[Category: Kumar, V.]]
[[Category: Kumar, V.]]
[[Category: Makde, R D.]]
[[Category: Makde, R D.]]
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[[Category: class-b bacterial acid phosphatase]]
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[[Category: Class-b bacterial acid phosphatase]]
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[[Category: cyclic-amp complex of apha protein]]
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[[Category: Cyclic-amp complex of apha protein]]
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[[Category: metalloenzyme]]
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[[Category: Metalloenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:12:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:29:32 2008''
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Revision as of 14:12, 3 May 2008

Image:1z5u.gif

Template:STRUCTURE 1z5u

Crystal structure of S. typhimurium AphA complexed with cyclic-AMP


Overview

The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.

About this Structure

1Z5U is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

Reference

Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135 Page seeded by OCA on Sat May 3 17:12:30 2008

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OCA

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