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2qnc
From Proteopedia
(Difference between revisions)
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==Crystal structure of T4 Endonuclease VII N62D mutant in complex with a DNA Holliday junction== | ==Crystal structure of T4 Endonuclease VII N62D mutant in complex with a DNA Holliday junction== | ||
| - | <StructureSection load='2qnc' size='340' side='right' caption='[[2qnc]], [[Resolution|resolution]] 3.10Å' scene=''> | + | <StructureSection load='2qnc' size='340' side='right'caption='[[2qnc]], [[Resolution|resolution]] 3.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2qnc]] is a 6 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2qnc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QNC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1e7d|1e7d]], [[1e7l|1e7l]], [[1en7|1en7]], [[2qnf|2qnf]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e7d|1e7d]], [[1e7l|1e7l]], [[1en7|1en7]], [[2qnf|2qnf]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">49 ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">49 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] </span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qnc OCA], [https://pdbe.org/2qnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qnc RCSB], [https://www.ebi.ac.uk/pdbsum/2qnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qnc ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/END7_BPT4 END7_BPT4]] Cleaves DNA cruciform and Y-structures as well as heteroduplex loops. Resolves Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches. |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qn/2qnc_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qn/2qnc_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 2qnc" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2qnc" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Bpt4]] | [[Category: Bpt4]] | ||
[[Category: Crossover junction endodeoxyribonuclease]] | [[Category: Crossover junction endodeoxyribonuclease]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Biertumpfel, C]] | [[Category: Biertumpfel, C]] | ||
[[Category: Suck, D]] | [[Category: Suck, D]] | ||
Revision as of 08:25, 25 June 2021
Crystal structure of T4 Endonuclease VII N62D mutant in complex with a DNA Holliday junction
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Categories: Bpt4 | Crossover junction endodeoxyribonuclease | Large Structures | Biertumpfel, C | Suck, D | Yang, W | Alternative initiation | Calcium | Endo vii | Four-way junction | Holliday junction | Hydrolase | Hydrolase-dna complex | Metal-binding | Resolvase | Resolving-enzyme | T4 endonuclease vii | Zinc
