|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal Structure of RelB-RelE antitoxin-toxin complex from Methanococcus jannaschii== | | ==Crystal Structure of RelB-RelE antitoxin-toxin complex from Methanococcus jannaschii== |
| - | <StructureSection load='3bpq' size='340' side='right' caption='[[3bpq]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='3bpq' size='340' side='right'caption='[[3bpq]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3bpq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BPQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BPQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3bpq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BPQ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">relB3, relB, MJ1103.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), MJ1103, relE, relE3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">relB3, relB, MJ1103.1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), MJ1103, relE, relE3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bpq OCA], [http://pdbe.org/3bpq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bpq RCSB], [http://www.ebi.ac.uk/pdbsum/3bpq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bpq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bpq OCA], [https://pdbe.org/3bpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bpq RCSB], [https://www.ebi.ac.uk/pdbsum/3bpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bpq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RELB3_METJA RELB3_METJA]] Antitoxin component of a toxin-antitoxin (TA) module. Probably neutralizes the toxic activity of cognate toxin RelE (By similarity).<ref>PMID:15718296</ref> [[http://www.uniprot.org/uniprot/RELE3_METJA RELE3_METJA]] Toxic component of a toxin-antitoxin (TA) module. Has RNase activity (By similarity). Is very toxic upon expression in E.coli. Its toxic activity is probably neutralized by the cognate antitoxin RelB3.<ref>PMID:15718296</ref> | + | [[https://www.uniprot.org/uniprot/RELB3_METJA RELB3_METJA]] Antitoxin component of a toxin-antitoxin (TA) module. Probably neutralizes the toxic activity of cognate toxin RelE (By similarity).<ref>PMID:15718296</ref> [[https://www.uniprot.org/uniprot/RELE3_METJA RELE3_METJA]] Toxic component of a toxin-antitoxin (TA) module. Has RNase activity (By similarity). Is very toxic upon expression in E.coli. Its toxic activity is probably neutralized by the cognate antitoxin RelB3.<ref>PMID:15718296</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bp/3bpq_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bp/3bpq_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| Line 32: |
Line 32: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Metja]] | | [[Category: Metja]] |
| | [[Category: Francuski, D]] | | [[Category: Francuski, D]] |
| Structural highlights
3bpq is a 4 chain structure with sequence from Metja. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Gene: | relB3, relB, MJ1103.1 (METJA), MJ1103, relE, relE3 (METJA) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[RELB3_METJA] Antitoxin component of a toxin-antitoxin (TA) module. Probably neutralizes the toxic activity of cognate toxin RelE (By similarity).[1] [RELE3_METJA] Toxic component of a toxin-antitoxin (TA) module. Has RNase activity (By similarity). Is very toxic upon expression in E.coli. Its toxic activity is probably neutralized by the cognate antitoxin RelB3.[2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Here we present the crystal structure of the Methanococcus jannaschii RelE-RelB (RelBE) toxin-antitoxin (TA) protein complex determined by the MIRAS (multiple isomorphous replacement with anomalous signal) method. The genes encoding this TA system are located in the chromosome of this archaeon and involved in stress response. RelE acts as an endoribonuclease that cleaves mRNA on the ribosome, and we compare the RelBE complex to the known structures of other TA systems belonging to this group and to endoribonucleases. M. jannaschii RelBE forms a heterotetramer with the antitoxin in the centre of the complex, a configuration that differs vastly from the heterotetramer structure of the previously published RelBE from another archaeon, Pyrococcus horikoshii. The long N-terminal alpha-helix of the tightly bound M. jannaschii antitoxin RelB covers the presumed active site of the toxin RelE that is formed by a central beta-sheet, a loop on one side and a C-terminal alpha-helix on the other side. The active site of the M. jannaschii toxin RelE harbours positive charges that are thought to neutralize the negative charges of the substrate mRNA, including Arg62 that was changed to Ser62 by the Escherichia coli expression system, thereby leading to inactive toxin RelE. Comparative studies suggest that Asp43 and His79 are also involved in the activity of the toxin.
Crystal structure of the antitoxin-toxin protein complex RelB-RelE from Methanococcus jannaschii.,Francuski D, Saenger W J Mol Biol. 2009 Nov 6;393(4):898-908. Epub 2009 Aug 25. PMID:19712680[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pandey DP, Gerdes K. Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes. Nucleic Acids Res. 2005 Feb 17;33(3):966-76. Print 2005. PMID:15718296 doi:http://dx.doi.org/10.1093/nar/gki201
- ↑ Pandey DP, Gerdes K. Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes. Nucleic Acids Res. 2005 Feb 17;33(3):966-76. Print 2005. PMID:15718296 doi:http://dx.doi.org/10.1093/nar/gki201
- ↑ Francuski D, Saenger W. Crystal structure of the antitoxin-toxin protein complex RelB-RelE from Methanococcus jannaschii. J Mol Biol. 2009 Nov 6;393(4):898-908. Epub 2009 Aug 25. PMID:19712680 doi:S0022-2836(09)01057-2
|
