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1zea
From Proteopedia
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'''Structure of the anti-cholera toxin antibody Fab fragment TE33 in complex with a D-peptide''' | '''Structure of the anti-cholera toxin antibody Fab fragment TE33 in complex with a D-peptide''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZEA OCA]. | |
==Reference== | ==Reference== | ||
Structure of an anti-cholera toxin antibody Fab in complex with an epitope-derived D-peptide: a case of polyspecific recognition., Scheerer P, Kramer A, Otte L, Seifert M, Wessner H, Scholz C, Krauss N, Schneider-Mergener J, Hohne W, J Mol Recognit. 2007 Jul-Aug;20(4):263-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17712773 17712773] | Structure of an anti-cholera toxin antibody Fab in complex with an epitope-derived D-peptide: a case of polyspecific recognition., Scheerer P, Kramer A, Otte L, Seifert M, Wessner H, Scholz C, Krauss N, Schneider-Mergener J, Hohne W, J Mol Recognit. 2007 Jul-Aug;20(4):263-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17712773 17712773] | ||
| - | [[Category: Mus musculus]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Hoehne, W.]] | [[Category: Hoehne, W.]] | ||
[[Category: Kramer, A.]] | [[Category: Kramer, A.]] | ||
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[[Category: Seifert, M.]] | [[Category: Seifert, M.]] | ||
[[Category: Wessner, H.]] | [[Category: Wessner, H.]] | ||
| - | [[Category: | + | [[Category: Anti-cholera toxin]] |
| - | [[Category: | + | [[Category: Antigen recognition]] |
| - | [[Category: | + | [[Category: Antigen-antibody complex]] |
| - | [[Category: | + | [[Category: Cross-reactivity]] |
| - | [[Category: | + | [[Category: Polyspecificity]] |
| - | [[Category: | + | [[Category: Substitution matrix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:31:12 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:31, 3 May 2008
Structure of the anti-cholera toxin antibody Fab fragment TE33 in complex with a D-peptide
Overview
The structure of a complex of the anti-cholera toxin antibody TE33 Fab (fragment antibody) with the D-peptide vpGsqhyds was solved to 1.78 A resolution. The D-peptide was derived from the linear L-peptide epitope VPGSQHIDS by a stepwise transformation. Despite the very similar amino acid sequence-the only difference is a tyrosine residue in position 7-there are marked differences in the individual positions with respect to their contribution to the peptide overall affinity as ascertained by a complete substitutional analysis. This is reflected by the X-ray structure of the TE33 Fab/D-peptide complex where there is an inverted orientation of the D-peptide as compared with the known structure of a corresponding complex containing the epitope L-peptide, with the side chains establishing different contacts within the binding site of TE33. The D- and L-peptide affinities are comparable and the surface areas buried by complex formation are almost the same. Thus the antibody TE33 provides a typical example for polyspecific binding behavior of IgG family antibodies.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure of an anti-cholera toxin antibody Fab in complex with an epitope-derived D-peptide: a case of polyspecific recognition., Scheerer P, Kramer A, Otte L, Seifert M, Wessner H, Scholz C, Krauss N, Schneider-Mergener J, Hohne W, J Mol Recognit. 2007 Jul-Aug;20(4):263-74. PMID:17712773 Page seeded by OCA on Sat May 3 17:31:12 2008
