1zem

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[[Image:1zem.gif|left|200px]]
[[Image:1zem.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1zem |SIZE=350|CAPTION= <scene name='initialview01'>1zem</scene>, resolution 1.9&Aring;
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The line below this paragraph, containing "STRUCTURE_1zem", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/D-xylulose_reductase D-xylulose reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.9 1.1.1.9] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= AB091690 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=442 Gluconobacter oxydans])
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-->
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|DOMAIN=
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{{STRUCTURE_1zem| PDB=1zem | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zem OCA], [http://www.ebi.ac.uk/pdbsum/1zem PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zem RCSB]</span>
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}}
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'''Crystal Structure of NAD+-Bound Xylitol Dehydrogenase'''
'''Crystal Structure of NAD+-Bound Xylitol Dehydrogenase'''
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[[Category: Elling, R A.]]
[[Category: Elling, R A.]]
[[Category: Wilson, D K.]]
[[Category: Wilson, D K.]]
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[[Category: dinucleotide-binding domain]]
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[[Category: Dinucleotide-binding domain]]
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[[Category: rossmann fold]]
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[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:31:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:34:57 2008''
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Revision as of 14:31, 3 May 2008

Image:1zem.gif

Template:STRUCTURE 1zem

Crystal Structure of NAD+-Bound Xylitol Dehydrogenase


Overview

Xylitol dehydrogenase (XDH) is one of several enzymes responsible for assimilating xylose into eukaryotic metabolism and is useful for fermentation of xylose contained in agricultural byproducts to produce ethanol. For efficient xylose utilization at high flux rates, cosubstrates should be recycled between the NAD+-specific XDH and the NADPH-preferring xylose reductase, another enzyme in the pathway. To understand and alter the cosubstrate specificity of XDH, we determined the crystal structure of the Gluconobacter oxydans holoenzyme to 1.9 angstroms resolution. The structure reveals that NAD+ specificity is largely conferred by Asp38, which interacts with the hydroxyls of the adenosine ribose. Met39 stacked under the purine ring and was also located near the 2' hydroxyl. Based on the location of these residues and on sequence alignments with related enzymes of various cosubstrate specificities, we constructed a double mutant (D38S/M39R) that was able to exclusively use NADP+, with no loss of activity.

About this Structure

1ZEM is a Single protein structure of sequence from Gluconobacter oxydans. Full crystallographic information is available from OCA.

Reference

Structure-guided engineering of xylitol dehydrogenase cosubstrate specificity., Ehrensberger AH, Elling RA, Wilson DK, Structure. 2006 Mar;14(3):567-75. PMID:16531240 Page seeded by OCA on Sat May 3 17:31:54 2008

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