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1zie

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[[Image:1zie.gif|left|200px]]
[[Image:1zie.gif|left|200px]]
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{{Structure
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{{STRUCTURE_1zie| PDB=1zie | SCENE= }}
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|RELATEDENTRY=[[1a5d|1A5D]], [[1zgt|1ZGT]], [[1ziq|1ZIQ]], [[1zir|1ZIR]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zie OCA], [http://www.ebi.ac.uk/pdbsum/1zie PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zie RCSB]</span>
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'''Hydrogenated gammaE crystallin in D2O solvent'''
'''Hydrogenated gammaE crystallin in D2O solvent'''
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[[Category: Timmins, P.]]
[[Category: Timmins, P.]]
[[Category: 4 greek key motif]]
[[Category: 4 greek key motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:39:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:37:07 2008''
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Revision as of 14:39, 3 May 2008

Image:1zie.gif

Template:STRUCTURE 1zie

Hydrogenated gammaE crystallin in D2O solvent


Overview

Rat gammaE-crystallin was overexpressed, purified under different labelling conditions and crystallized and X-ray data were collected at resolutions between 1.71 and 1.36 A. The structures were determined by molecular replacement. In these structures, the cd loop of the Greek-key motif 3, which is the major structural key motif of the two phase-transition groups of gamma-crystallins, presents a double conformation. The influence of the perdeuteration on the protein structure was determined by comparison of the atomic positions and temperature factors of the different models. The perdeuterated proteins have a similar structure to their hydrogenated counterparts, but partial or full deuteration may have some effect on the atomic B-factor values.

About this Structure

1ZIE is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O., Artero JB, Hartlein M, McSweeney S, Timmins P, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1541-9. Epub 2005, Oct 19. PMID:16239733 Page seeded by OCA on Sat May 3 17:39:48 2008

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