1zkk

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[[Image:1zkk.gif|left|200px]]
[[Image:1zkk.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1zkk |SIZE=350|CAPTION= <scene name='initialview01'>1zkk</scene>, resolution 1.45&Aring;
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The line below this paragraph, containing "STRUCTURE_1zkk", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= SET8, PRSET7, SET07 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1zkk| PDB=1zkk | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zkk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zkk OCA], [http://www.ebi.ac.uk/pdbsum/1zkk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zkk RCSB]</span>
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}}
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'''Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy'''
'''Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy'''
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[[Category: Couture, J F.]]
[[Category: Couture, J F.]]
[[Category: Trievel, R C.]]
[[Category: Trievel, R C.]]
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[[Category: beta-sheet]]
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[[Category: Beta-sheet]]
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[[Category: histone h4]]
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[[Category: Histone h4]]
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[[Category: pseudo-knot]]
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[[Category: Pseudo-knot]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:44:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:37:57 2008''
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Revision as of 14:44, 3 May 2008

Image:1zkk.gif

Template:STRUCTURE 1zkk

Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy


Overview

SET8 (also known as PR-SET7) is a histone H4-Lys-20-specific methyltransferase that is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. Herein we report the crystal structure of human SET8 (hSET8) bound to a histone H4 peptide bearing Lys-20 and the product cofactor S-adenosylhomocysteine. Histone H4 intercalates in the substrate-binding cleft as an extended parallel beta-strand. Residues preceding Lys-20 in H4 engage in an extensive array of salt bridge, hydrogen bond, and van der Waals interactions with hSET8, while the C-terminal residues bind through predominantly hydrophobic interactions. Mutational analysis of both the substrate-binding cleft and histone H4 reveals that interactions with residues in the N and C termini of the H4 peptide are critical for conferring substrate specificity. Finally, analysis of the product specificity indicates that hSET8 is a monomethylase, consistent with its role in the maintenance of Lys-20 monomethylation during cell division.

About this Structure

1ZKK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of SET8, a histone H4 Lys-20 methyltransferase., Couture JF, Collazo E, Brunzelle JS, Trievel RC, Genes Dev. 2005 Jun 15;19(12):1455-65. Epub 2005 Jun 2. PMID:15933070 Page seeded by OCA on Sat May 3 17:44:22 2008

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