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1zkk
From Proteopedia
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[[Image:1zkk.gif|left|200px]] | [[Image:1zkk.gif|left|200px]] | ||
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'''Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy''' | '''Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy''' | ||
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[[Category: Couture, J F.]] | [[Category: Couture, J F.]] | ||
[[Category: Trievel, R C.]] | [[Category: Trievel, R C.]] | ||
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| - | [[Category: | + | [[Category: Histone h4]] |
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:44:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:44, 3 May 2008
Crystal structure of hSET8 in ternary complex with H4 peptide (16-24) and AdoHcy
Overview
SET8 (also known as PR-SET7) is a histone H4-Lys-20-specific methyltransferase that is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. Herein we report the crystal structure of human SET8 (hSET8) bound to a histone H4 peptide bearing Lys-20 and the product cofactor S-adenosylhomocysteine. Histone H4 intercalates in the substrate-binding cleft as an extended parallel beta-strand. Residues preceding Lys-20 in H4 engage in an extensive array of salt bridge, hydrogen bond, and van der Waals interactions with hSET8, while the C-terminal residues bind through predominantly hydrophobic interactions. Mutational analysis of both the substrate-binding cleft and histone H4 reveals that interactions with residues in the N and C termini of the H4 peptide are critical for conferring substrate specificity. Finally, analysis of the product specificity indicates that hSET8 is a monomethylase, consistent with its role in the maintenance of Lys-20 monomethylation during cell division.
About this Structure
1ZKK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of SET8, a histone H4 Lys-20 methyltransferase., Couture JF, Collazo E, Brunzelle JS, Trievel RC, Genes Dev. 2005 Jun 15;19(12):1455-65. Epub 2005 Jun 2. PMID:15933070 Page seeded by OCA on Sat May 3 17:44:22 2008
