1zme

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[[Image:1zme.gif|left|200px]]
[[Image:1zme.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1zme", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=5IU:5-IODO-2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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{{STRUCTURE_1zme| PDB=1zme | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zme OCA], [http://www.ebi.ac.uk/pdbsum/1zme PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zme RCSB]</span>
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'''CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX'''
'''CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX'''
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[[Category: Marmorstein, R.]]
[[Category: Marmorstein, R.]]
[[Category: Swaminathan, K.]]
[[Category: Swaminathan, K.]]
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[[Category: binuclear cluster]]
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[[Category: Binuclear cluster]]
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[[Category: complex (transcription regulation/dna)]]
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[[Category: Put3]]
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[[Category: put3]]
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[[Category: Transcription factor]]
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[[Category: transcription factor]]
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[[Category: Zn2cys6]]
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[[Category: zn2cys6]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:48:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:38:43 2008''
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Revision as of 14:48, 3 May 2008

Image:1zme.gif

Template:STRUCTURE 1zme

CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX


Overview

PUT3 is a member of a family of at least 79 fungal transcription factors that contain a six-cysteine, two-zinc domain called a 'Zn2Cys6 binuclear cluster'. We have determined the crystal structure of the DNA binding region from the PUT3 protein bound to its cognate DNA target. The structure reveals that the PUT3 homodimer is bound asymmetrically to the DNA site. This asymmetry orients a beta-strand from one protein subunit into the minor groove of the DNA resulting in a partial amino acid-base pair intercalation and extensive direct and water-mediated protein interactions with the minor groove of the DNA. These interactions facilitate a sequence dependent kink at the centre of the DNA site and specify the intervening base pairs separating two DNA half-sites that are contacted in the DNA major groove. A comparison with the GAL4-DNA and PPR1-DNA complexes shows how a family of related DNA binding proteins can use a diverse set of mechanisms to discriminate between the base pairs separating conserved DNA half-sites.

About this Structure

1ZME is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of a PUT3-DNA complex reveals a novel mechanism for DNA recognition by a protein containing a Zn2Cys6 binuclear cluster., Swaminathan K, Flynn P, Reece RJ, Marmorstein R, Nat Struct Biol. 1997 Sep;4(9):751-9. PMID:9303004 Page seeded by OCA on Sat May 3 17:48:27 2008

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