3fmr

<StructureSection load='3fmr' size='340' side='right' caption='[[3fmr]], [[Resolution|resolution]] 2.89&Aring;' scene=''>

<table><tr><td colspan='2'>[[3fmr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enccn Enccn]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3d0d 3d0d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FMR FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TN4:(1R,2S,3S,4R)-4-HYDROXY-2-METHOXY-4-METHYL-3-[(2R,3R)-2-METHYL-3-(3-METHYLBUT-2-EN-1-YL)OXIRAN-2-YL]CYCLOHEXYL+(CHLOROACETYL)CARBAMATE'>TN4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fmr OCA], [http://pdbe.org/3fmr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fmr RCSB], [http://www.ebi.ac.uk/pdbsum/3fmr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fmr ProSAT]</span></td></tr>

== Function ==

[[http://www.uniprot.org/uniprot/AMPM2_ENCCU AMPM2_ENCCU]] Removes the N-terminal methionine from nascent proteins.

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/3fmr_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

Microsporidia are protists that have been reported to cause infections in both vertebrates and invertebrates. They have emerged as human pathogens particularly in patients that are immunosuppressed and cases of gastrointestinal infection, encephalitis, keratitis, sinusitis, myositis and disseminated infection are well described in the literature. While benzimidazoles are active against many species of microsporidia, these drugs do not have significant activity against Enterocytozoon bieneusi. Fumagillin and its analogues have been demonstrated to have activity invitro and in animal models of microsporidiosis and human infections due to E. bieneusi. Fumagillin and its analogues inhibit methionine aminopeptidase type 2. Encephalitozoon cuniculi MetAP2 (EcMetAP2) was cloned and expressed as an active enzyme using a baculovirus system. The crystal structure of EcMetAP2 was determined with and without the bound inhibitors fumagillin and TNP-470. This structure classifies EcMetAP2 as a member of the MetAP2c family. The EcMetAP2 structure was used to generate a homology model of the E. bieneusi MetAP2. Comparison of microsporidian MetAP2 structures with human MetAP2 provides insights into the design of inhibitors that might exhibit specificity for microsporidian MetAP2.

 

== References ==

[[Category: Enccn]]

[[Category: Methionyl aminopeptidase]]

[[Category: Adams, J]]

[[Category: Almo, S C]]

[[Category: Alvarado, J J]]

[[Category: Burley, S K]]

[[Category: Structural genomic]]

[[Category: Russell, M]]

[[Category: Toro, R]]

[[Category: Weiss, L M]]

[[Category: Zhang, A]]

[[Category: PSI, Protein structure initiative]]