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| | ==Heterodimer crystal structure of geranylgeranyl diphosphate synthases 1 with GGPPS Recruiting Protein(OsGRP) from Oryza sativa== | | ==Heterodimer crystal structure of geranylgeranyl diphosphate synthases 1 with GGPPS Recruiting Protein(OsGRP) from Oryza sativa== |
| - | <StructureSection load='5xn6' size='340' side='right' caption='[[5xn6]], [[Resolution|resolution]] 3.60Å' scene=''> | + | <StructureSection load='5xn6' size='340' side='right'caption='[[5xn6]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5xn6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XN6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XN6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xn6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XN6 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Os07g0580900, OJ1301_C12.3, OSNPB_070580900, P0453G03.27 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice]), Os02g0668100, OJ1725_H08.13, OSNPB_020668100 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.598Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xn6 OCA], [http://pdbe.org/5xn6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xn6 RCSB], [http://www.ebi.ac.uk/pdbsum/5xn6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xn6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xn6 OCA], [https://pdbe.org/5xn6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xn6 RCSB], [https://www.ebi.ac.uk/pdbsum/5xn6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xn6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7XI92_ORYSJ Q7XI92_ORYSJ] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Japanese rice]] | + | [[Category: Large Structures]] |
| - | [[Category: Lu, S]] | + | [[Category: Oryza sativa Japonica Group]] |
| - | [[Category: Wang, C]] | + | [[Category: Lu S]] |
| - | [[Category: Zhang, P]] | + | [[Category: Wang C]] |
| - | [[Category: Zhou, F]] | + | [[Category: Zhang P]] |
| - | [[Category: Heterodimer]]
| + | [[Category: Zhou F]] |
| - | [[Category: Osggpps1]]
| + | |
| - | [[Category: Osgrp]]
| + | |
| - | [[Category: Small-subunit]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
Q7XI92_ORYSJ
Publication Abstract from PubMed
In plants, geranylgeranyl diphosphate (GGPP) is produced by plastidic GGPP synthase (GGPPS) and serves as a precursor for vital metabolic branches, including chlorophyll, carotenoid, and gibberellin biosynthesis. However, molecular mechanisms regulating GGPP allocation among these biosynthetic pathways localized in the same subcellular compartment are largely unknown. We found that rice contains only one functionally active GGPPS, OsGGPPS1, in chloroplasts. A functionally active homodimeric enzyme composed of two OsGGPPS1 subunits is located in the stroma. In thylakoid membranes, however, the GGPPS activity resides in a heterodimeric enzyme composed of one OsGGPPS1 subunit and GGPPS recruiting protein (OsGRP). OsGRP is structurally most similar to members of the geranyl diphosphate synthase small subunit type II subfamily. In contrast to members of this subfamily, OsGRP enhances OsGGPPS1 catalytic efficiency and specificity of GGPP production on interaction with OsGGPPS1. Structural biology and protein interaction analyses demonstrate that affinity between OsGRP and OsGGPPS1 is stronger than between two OsGGPPS1 molecules in homodimers. OsGRP determines OsGGPPS1 suborganellar localization and directs it to a large protein complex in thylakoid membranes, consisting of geranylgeranyl reductase (OsGGR), light-harvesting-like protein 3 (OsLIL3), protochlorophyllide oxidoreductase (OsPORB), and chlorophyll synthase (OsCHLG). Taken together, genetic and biochemical analyses suggest OsGRP functions in recruiting OsGGPPS1 from the stroma toward thylakoid membranes, thus providing a mechanism to control GGPP flux toward chlorophyll biosynthesis.
A recruiting protein of geranylgeranyl diphosphate synthase controls metabolic flux toward chlorophyll biosynthesis in rice.,Zhou F, Wang CY, Gutensohn M, Jiang L, Zhang P, Zhang D, Dudareva N, Lu S Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):6866-6871. doi:, 10.1073/pnas.1705689114. Epub 2017 Jun 12. PMID:28607067[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhou F, Wang CY, Gutensohn M, Jiang L, Zhang P, Zhang D, Dudareva N, Lu S. A recruiting protein of geranylgeranyl diphosphate synthase controls metabolic flux toward chlorophyll biosynthesis in rice. Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):6866-6871. doi:, 10.1073/pnas.1705689114. Epub 2017 Jun 12. PMID:28607067 doi:http://dx.doi.org/10.1073/pnas.1705689114
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