1zyc
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1zyc.gif|left|200px]] | [[Image:1zyc.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1zyc", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1zyc| PDB=1zyc | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal Structure of eIF2alpha Protein Kinase GCN2: Wild-Type in Apo Form.''' | '''Crystal Structure of eIF2alpha Protein Kinase GCN2: Wild-Type in Apo Form.''' | ||
| Line 31: | Line 28: | ||
[[Category: Qiu, H.]] | [[Category: Qiu, H.]] | ||
[[Category: Roll-Mecak, A.]] | [[Category: Roll-Mecak, A.]] | ||
| - | [[Category: | + | [[Category: Amino-acid starvation]] |
| - | [[Category: | + | [[Category: Eif2alpha kinase]] |
| - | [[Category: | + | [[Category: Protein kinase]] |
| - | [[Category: | + | [[Category: Signal transduction]] |
| - | [[Category: | + | [[Category: Starvation stress response]] |
| - | [[Category: | + | [[Category: Translation regulator]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:13:54 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:13, 3 May 2008
Crystal Structure of eIF2alpha Protein Kinase GCN2: Wild-Type in Apo Form.
Overview
The GCN2 protein kinase coordinates protein synthesis with levels of amino acid stores by phosphorylating eukaryotic translation initiation factor 2. The autoinhibited form of GCN2 is activated in cells starved of amino acids by binding of uncharged tRNA to a histidyl-tRNA synthetase-like domain. Replacement of Arg-794 with Gly in the PK domain (R794G) activates GCN2 independently of tRNA binding. Crystal structures of the GCN2 protein kinase domain have been determined for wild-type and R794G mutant forms in the apo state and bound to ATP/AMPPNP. These structures reveal that GCN2 autoinhibition results from stabilization of a closed conformation that restricts ATP binding. The R794G mutant shows increased flexibility in the hinge region connecting the N- and C-lobes, resulting from loss of multiple interactions involving Arg794. This conformational change is associated with intradomain movement that enhances ATP binding and hydrolysis. We propose that intramolecular interactions following tRNA binding remodel the hinge region in a manner similar to the mechanism of enzyme activation elicited by the R794G mutation.
About this Structure
1ZYC is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural basis for autoinhibition and mutational activation of eukaryotic initiation factor 2alpha protein kinase GCN2., Padyana AK, Qiu H, Roll-Mecak A, Hinnebusch AG, Burley SK, J Biol Chem. 2005 Aug 12;280(32):29289-99. Epub 2005 Jun 17. PMID:15964839 Page seeded by OCA on Sat May 3 18:13:54 2008
Categories: Non-specific serine/threonine protein kinase | Saccharomyces cerevisiae | Single protein | Burley, S K. | Hinnebusch, A G. | Padyana, A K. | Qiu, H. | Roll-Mecak, A. | Amino-acid starvation | Eif2alpha kinase | Protein kinase | Signal transduction | Starvation stress response | Translation regulator
