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5ii9

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Revision as of 07:19, 3 June 2020

Monoclinic crystal structure of red abalone lysin at 2.11 A resolution

Structural highlights

5ii9 is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1lis, 2lis, 1lyn, 2lyn, 5ii7, 5ii8, 5iia, 5iib
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ELYS_HALRU] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.

Publication Abstract from PubMed

Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1-3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here. Whereas sequence-divergent repeat 1 does not bind lysin, repeat 3 binds it non-species specifically via a high-affinity, largely hydrophobic interface. Due to its intermediate binding affinity, repeat 2 selectively interacts with lysin from the same species. Exposure of a highly positively charged surface of VERL-bound lysin suggests that complex formation both disrupts the organization of egg coat filaments and triggers their electrostatic repulsion, thereby opening a hole for sperm penetration and fusion.

Structural Basis of Egg Coat-Sperm Recognition at Fertilization.,Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L. Structural Basis of Egg Coat-Sperm Recognition at Fertilization. Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512 doi:http://dx.doi.org/10.1016/j.cell.2017.05.033

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ii9"

@@ Line 1: / Line 1: @@
 ==Monoclinic crystal structure of red abalone lysin at 2.11 A resolution==
-<StructureSection load='5ii9' size='340' side='right' caption='[[5ii9]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
+<StructureSection load='5ii9' size='340' side='right'caption='[[5ii9]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ii9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5II9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5II9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ii9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5II9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5II9 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lis|1lis]], [[2lis|2lis]], [[1lyn|1lyn]], [[2lyn|2lyn]], [[5ii7|5ii7]], [[5ii8|5ii8]], [[5iia|5iia]], [[5iib|5iib]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ii9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ii9 OCA], [http://pdbe.org/5ii9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ii9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ii9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ii9 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ii9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ii9 OCA], [http://pdbe.org/5ii9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ii9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ii9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ii9 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 5ii9" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lysin|Lysin]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Al-Hosseini, H Sadat]]
 [[Category: Jovine, L]]

5ii9

From Proteopedia

Revision as of 07:19, 3 June 2020

Monoclinic crystal structure of red abalone lysin at 2.11 A resolution

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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