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| | ==Crystal structure of PXY-CLE41-SERK2== | | ==Crystal structure of PXY-CLE41-SERK2== |
| - | <StructureSection load='5gqr' size='340' side='right' caption='[[5gqr]], [[Resolution|resolution]] 3.50Å' scene=''> | + | <StructureSection load='5gqr' size='340' side='right'caption='[[5gqr]], [[Resolution|resolution]] 3.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gqr]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GQR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gqr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabideae Arabideae] and [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GQR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TDR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), SERK2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gqr OCA], [https://pdbe.org/5gqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gqr RCSB], [https://www.ebi.ac.uk/pdbsum/5gqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gqr ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gqr OCA], [http://pdbe.org/5gqr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gqr RCSB], [http://www.ebi.ac.uk/pdbsum/5gqr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gqr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TDR_ARATH TDR_ARATH]] Acts with CLE41p and CLE44p peptides as a ligand-receptor pair in a signal transduction pathway involved in the regulation of procambium maintenance and polarity during vascular-tissue development. Mediates repression of tracheary element differentiation and the promotion of procambial cells formation and polar division adjacent to phloem cells in the veins.<ref>PMID:17570668</ref> <ref>PMID:18812507</ref> [[http://www.uniprot.org/uniprot/SERK2_ARATH SERK2_ARATH]] Serine/threonine-kinase involved in brassinosteroid-dependent and -independent signaling pathways. Acts redundantly with SERK1 as a control point for sporophytic development controlling male gametophyte production.<ref>PMID:18667726</ref> | + | [https://www.uniprot.org/uniprot/TDR_ARATH TDR_ARATH] Acts with CLE41p and CLE44p peptides as a ligand-receptor pair in a signal transduction pathway involved in the regulation of procambium maintenance and polarity during vascular-tissue development. Mediates repression of tracheary element differentiation and the promotion of procambial cells formation and polar division adjacent to phloem cells in the veins.<ref>PMID:17570668</ref> <ref>PMID:18812507</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabideae]] |
| - | [[Category: Non-specific serine/threonine protein kinase]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Chai, J J]] | + | [[Category: Large Structures]] |
| - | [[Category: Zhang, H Q]] | + | [[Category: Chai JJ]] |
| - | [[Category: Cle peptide]] | + | [[Category: Zhang HQ]] |
| - | [[Category: Leucine rich repeat]]
| + | |
| - | [[Category: Meristem cell proliferation]]
| + | |
| - | [[Category: Serk]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
TDR_ARATH Acts with CLE41p and CLE44p peptides as a ligand-receptor pair in a signal transduction pathway involved in the regulation of procambium maintenance and polarity during vascular-tissue development. Mediates repression of tracheary element differentiation and the promotion of procambial cells formation and polar division adjacent to phloem cells in the veins.[1] [2]
Publication Abstract from PubMed
In Arabidopsis, the CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides play important roles in regulating proliferation and differentiation of plant-specific stem cells. Although receptors of CLEs are reported to be leucine-rich repeat receptor kinases, the mechanisms underlying CLE-induced receptor activation remain largely unknown. Here we show that SOMATIC EMBRYOGENESIS RECEPTOR KINASEs (SERKs) serve as co-receptors in CLE41/TDIF-PXY signaling to regulate plant vascular development. TDIF induces interaction of its receptor PXY with SERKs in vitro and in vivo. Furthermore, the serk1-1 serk2-1 bak1-5 mutant plants are less sensitive to TDIF, phenocopying the pxy mutant with a compromised promotion of procambial cell proliferation. Crystal structure of the PXY-TDIF-SERK2 complex reveals that the last amino acid of TDIF conserved among CLEs and other evolutionary-related peptides is important for the interaction between SERK2 and PXY. Taken together, our current study identifies SERKs as signaling components of the TDIF-PXY pathway and suggests a conserved activation mechanism of CLE receptors.
SERK Family Receptor-like Kinases Function as Co-receptors with PXY for Plant Vascular Development.,Zhang H, Lin X, Han Z, Wang J, Qu LJ, Chai J Mol Plant. 2016 Oct 10;9(10):1406-1414. doi: 10.1016/j.molp.2016.07.004. Epub, 2016 Jul 21. PMID:27449136[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fisher K, Turner S. PXY, a receptor-like kinase essential for maintaining polarity during plant vascular-tissue development. Curr Biol. 2007 Jun 19;17(12):1061-6. PMID:17570668 doi:http://dx.doi.org/10.1016/j.cub.2007.05.049
- ↑ Hirakawa Y, Shinohara H, Kondo Y, Inoue A, Nakanomyo I, Ogawa M, Sawa S, Ohashi-Ito K, Matsubayashi Y, Fukuda H. Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15208-13. doi:, 10.1073/pnas.0808444105. Epub 2008 Sep 23. PMID:18812507 doi:http://dx.doi.org/10.1073/pnas.0808444105
- ↑ Zhang H, Lin X, Han Z, Wang J, Qu LJ, Chai J. SERK Family Receptor-like Kinases Function as Co-receptors with PXY for Plant Vascular Development. Mol Plant. 2016 Oct 10;9(10):1406-1414. doi: 10.1016/j.molp.2016.07.004. Epub, 2016 Jul 21. PMID:27449136 doi:http://dx.doi.org/10.1016/j.molp.2016.07.004
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