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296d
From Proteopedia
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[[Image:296d.gif|left|200px]] | [[Image:296d.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_296d| PDB=296d | SCENE= }} | |
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'''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX''' | '''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA]. | |
==Reference== | ==Reference== | ||
Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7515488 7515488] | Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7515488 7515488] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Brown, D G.]] | [[Category: Brown, D G.]] | ||
[[Category: Neidle, S.]] | [[Category: Neidle, S.]] | ||
[[Category: Skelly, J V.]] | [[Category: Skelly, J V.]] | ||
[[Category: Spink, N.]] | [[Category: Spink, N.]] | ||
| - | [[Category: | + | [[Category: B-dna]] |
| - | [[Category: | + | [[Category: Complexed with drug]] |
| - | [[Category: | + | [[Category: Double helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:26:08 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:26, 3 May 2008
SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX
Overview
The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.
About this Structure
Full crystallographic information is available from OCA.
Reference
Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:7515488 Page seeded by OCA on Sat May 3 18:26:08 2008
