6b9s
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==MPnS crystallized in the absence of substrate== | |
| + | <StructureSection load='6b9s' size='340' side='right' caption='[[6b9s]], [[Resolution|resolution]] 2.37Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6b9s]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B9S FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylphosphonate_synthase Methylphosphonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.73 1.13.11.73] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b9s OCA], [http://pdbe.org/6b9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b9s RCSB], [http://www.ebi.ac.uk/pdbsum/6b9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b9s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/MPNS_NITMS MPNS_NITMS]] Catalyzes the conversion of 2-hydroxyethylphosphonate into methylphosphonate in the methylphosphonate biosynthesis pathway.<ref>PMID:22936780</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Methylphosphonate synthase (MPnS) produces methylphosphonate, a metabolic precursor to methane in the upper ocean. Here, we determine a 2.35-angstrom resolution structure of MPnS and discover that it has an unusual 2-histidine-1-glutamine iron-coordinating triad. We further solve the structure of a related enzyme, hydroxyethylphosphonate dioxygenase from Streptomyces albus (SaHEPD), and find that it displays the same motif. SaHEPD can be converted into an MPnS by mutation of glutamine-adjacent residues, identifying the molecular requirements for methylphosphonate synthesis. Using these sequence markers, we find numerous putative MPnSs in marine microbiomes and confirm that MPnS is present in the abundant Pelagibacter ubique. The ubiquity of MPnS-containing microbes supports the proposal that methylphosphonate is a source of methane in the upper, aerobic ocean, where phosphorus-starved microbes catabolize methylphosphonate for its phosphorus. | ||
| - | + | Structural basis for methylphosphonate biosynthesis.,Born DA, Ulrich EC, Ju KS, Peck SC, van der Donk WA, Drennan CL Science. 2017 Dec 8;358(6368):1336-1339. doi: 10.1126/science.aao3435. PMID:29217579<ref>PMID:29217579</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6b9s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Methylphosphonate synthase]] | ||
| + | [[Category: Born, D A]] | ||
| + | [[Category: Drennan, C L]] | ||
| + | [[Category: Iron]] | ||
| + | [[Category: Methylphosphonate]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Phosphonate]] | ||
Revision as of 06:24, 20 December 2017
MPnS crystallized in the absence of substrate
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