2a1s

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[[Image:2a1s.gif|left|200px]]
[[Image:2a1s.gif|left|200px]]
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{{Structure
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|PDB= 2a1s |SIZE=350|CAPTION= <scene name='initialview01'>2a1s</scene>, resolution 2.60&Aring;
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The line below this paragraph, containing "STRUCTURE_2a1s", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(A)-specific_ribonuclease Poly(A)-specific ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.13.4 3.1.13.4] </span>
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{{STRUCTURE_2a1s| PDB=2a1s | SCENE= }}
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|RELATEDENTRY=[[2a1r|2A1R]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a1s OCA], [http://www.ebi.ac.uk/pdbsum/2a1s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a1s RCSB]</span>
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'''Crystal structure of native PARN nuclease domain'''
'''Crystal structure of native PARN nuclease domain'''
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[[Category: Exosomes]]
[[Category: Exosomes]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Poly(A)-specific ribonuclease]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Song, H.]]
[[Category: Song, H.]]
[[Category: Wu, M.]]
[[Category: Wu, M.]]
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[[Category: dedd]]
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[[Category: Dedd]]
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[[Category: nuclease domain]]
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[[Category: Nuclease domain]]
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[[Category: parn]]
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[[Category: Parn]]
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[[Category: r3h]]
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[[Category: R3h]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:29:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:46:39 2008''
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Revision as of 15:29, 3 May 2008

Image:2a1s.gif

Template:STRUCTURE 2a1s

Crystal structure of native PARN nuclease domain


Overview

Poly(A)-specific ribonuclease (PARN) is a processive, poly(A)-specific 3' exoribonuclease. The crystal structure of C-terminal truncated human PARN determined in two states (free and RNA-bound forms) reveals that PARNn is folded into two domains, an R3H domain and a nuclease domain similar to those of Pop2p and epsilon186. The high similarity of the active site structures of PARNn and epsilon186 suggests that they may have a similar catalytic mechanism. PARNn forms a tight homodimer, with the R3H domain of one subunit partially enclosing the active site of the other subunit and poly(A) bound in a deep cavity of its nuclease domain in a sequence-nonspecific manner. The R3H domain and, possibly, the cap-binding domain are involved in poly(A) binding but these domains alone do not appear to contribute to poly(A) specificity. Mutations disrupting dimerization abolish both the enzymatic and RNA-binding activities, suggesting that the PARN dimer is a structural and functional unit. The cap-binding domain may act in concert with the R3H domain to amplify the processivity of PARN.

About this Structure

2A1S is a Single protein structure of sequence from Homo sapiens. The following page contains interesting information on the relation of 2A1S with [Exosomes]. Full crystallographic information is available from OCA.

Reference

Structural insight into poly(A) binding and catalytic mechanism of human PARN., Wu M, Reuter M, Lilie H, Liu Y, Wahle E, Song H, EMBO J. 2005 Dec 7;24(23):4082-93. Epub 2005 Nov 10. PMID:16281054 Page seeded by OCA on Sat May 3 18:29:47 2008

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