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2a1u
From Proteopedia
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[[Image:2a1u.gif|left|200px]] | [[Image:2a1u.gif|left|200px]] | ||
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'''Crystal structure of the human ETF E165betaA mutant''' | '''Crystal structure of the human ETF E165betaA mutant''' | ||
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[[Category: Thiel, A Van.]] | [[Category: Thiel, A Van.]] | ||
[[Category: Toogood, H S.]] | [[Category: Toogood, H S.]] | ||
| - | [[Category: | + | [[Category: Conformational sampling]] |
| - | [[Category: | + | [[Category: Electron transfer]] |
| - | [[Category: | + | [[Category: Mobile domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:29:50 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:29, 3 May 2008
Crystal structure of the human ETF E165betaA mutant
Overview
Crystal structures of protein complexes with electron-transferring flavoprotein (ETF) have revealed a dual protein-protein interface with one region serving as anchor while the ETF FAD domain samples available space within the complex. We show that mutation of the conserved Glu-165beta in human ETF leads to drastically modulated rates of interprotein electron transfer with both medium chain acyl-CoA dehydrogenase and dimethylglycine dehydrogenase. The crystal structure of free E165betaA ETF is essentially identical to that of wild-type ETF, but the crystal structure of the E165betaA ETF.medium chain acyl-CoA dehydrogenase complex reveals clear electron density for the FAD domain in a position optimal for fast interprotein electron transfer. Based on our observations, we present a dynamic multistate model for conformational sampling that for the wild-type ETF. medium chain acyl-CoA dehydrogenase complex involves random motion between three distinct positions for the ETF FAD domain. ETF Glu-165beta plays a key role in stabilizing positions incompatible with fast interprotein electron transfer, thus ensuring high rates of complex dissociation.
About this Structure
2A1U is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Stabilization of non-productive conformations underpins rapid electron transfer to electron-transferring flavoprotein., Toogood HS, van Thiel A, Scrutton NS, Leys D, J Biol Chem. 2005 Aug 26;280(34):30361-6. Epub 2005 Jun 23. PMID:15975918 Page seeded by OCA on Sat May 3 18:29:50 2008
