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| | ==Crystal structure of Sugen kinase 223== | | ==Crystal structure of Sugen kinase 223== |
| - | <StructureSection load='5ve6' size='340' side='right' caption='[[5ve6]], [[Resolution|resolution]] 2.95Å' scene=''> | + | <StructureSection load='5ve6' size='340' side='right'caption='[[5ve6]], [[Resolution|resolution]] 2.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ve6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VE6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ve6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VE6 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SGK223 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.953Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_protein-tyrosine_kinase Non-specific protein-tyrosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.2 2.7.10.2] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ve6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ve6 OCA], [https://pdbe.org/5ve6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ve6 RCSB], [https://www.ebi.ac.uk/pdbsum/5ve6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ve6 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ve6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ve6 OCA], [http://pdbe.org/5ve6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ve6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ve6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ve6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/PRAG1_HUMAN PRAG1_HUMAN] |
| - | The mammalian pseudokinase SgK223, and its structurally related homologue SgK269, are oncogenic scaffolds that nucleate the assembly of specific signalling complexes and regulate tyrosine kinase signalling. Both SgK223 and SgK269 form homo- and hetero-oligomers, a mechanism that underpins a diversity of signalling outputs. However, mechanistic insights into SgK223 and SgK269 homo- and heterotypic association are lacking. Here we present the crystal structure of SgK223 pseudokinase domain and its adjacent N- and C-terminal helices. The structure reveals how the N- and C-regulatory helices engage in a novel fold to mediate the assembly of a high-affinity dimer. In addition, we identified regulatory interfaces on the pseudokinase domain required for the self-assembly of large open-ended oligomers. This study highlights the diversity in how the kinase fold mediates non-catalytic functions and provides mechanistic insights into how the assembly of these two oncogenic scaffolds is achieved in order to regulate signalling output.
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| - | Structure of SgK223 pseudokinase reveals novel mechanisms of homotypic and heterotypic association.,Patel O, Griffin MDW, Panjikar S, Dai W, Ma X, Chan H, Zheng C, Kropp A, Murphy JM, Daly RJ, Lucet IS Nat Commun. 2017 Oct 27;8(1):1157. doi: 10.1038/s41467-017-01279-9. PMID:29079850<ref>PMID:29079850</ref>
| + | ==See Also== |
| - | | + | *[[Tyrosine kinase 3D structures|Tyrosine kinase 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5ve6" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Non-specific protein-tyrosine kinase]] | + | [[Category: Large Structures]] |
| - | [[Category: Lucet, I]] | + | [[Category: Lucet I]] |
| - | [[Category: Panjikar, S]] | + | [[Category: Panjikar S]] |
| - | [[Category: Patel, O]] | + | [[Category: Patel O]] |
| - | [[Category: Kinase]]
| + | |
| - | [[Category: Pseudokinase]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
