This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2a5d
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2a5d.gif|left|200px]] | [[Image:2a5d.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2a5d", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_2a5d| PDB=2a5d | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structural basis for the activation of cholera toxin by human ARF6-GTP''' | '''Structural basis for the activation of cholera toxin by human ARF6-GTP''' | ||
| Line 24: | Line 21: | ||
Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16099990 16099990] | Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16099990 16099990] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: NAD(+)--diphthamide ADP-ribosyltransferase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Vibrio cholerae]] | [[Category: Vibrio cholerae]] | ||
| Line 31: | Line 27: | ||
[[Category: Jobling, M G.]] | [[Category: Jobling, M G.]] | ||
[[Category: Neal, C J.O.]] | [[Category: Neal, C J.O.]] | ||
| - | [[Category: | + | [[Category: Protein transport/transferase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:37:40 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:37, 3 May 2008
Structural basis for the activation of cholera toxin by human ARF6-GTP
Overview
The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs.
About this Structure
2A5D is a Protein complex structure of sequences from Homo sapiens and Vibrio cholerae. Full crystallographic information is available from OCA.
Reference
Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:16099990 Page seeded by OCA on Sat May 3 18:37:40 2008
