2a5f

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[[Image:2a5f.gif|left|200px]]
[[Image:2a5f.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2a5f |SIZE=350|CAPTION= <scene name='initialview01'>2a5f</scene>, resolution 2.02&Aring;
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The line below this paragraph, containing "STRUCTURE_2a5f", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ARF6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ctxA, toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
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-->
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|DOMAIN=
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{{STRUCTURE_2a5f| PDB=2a5f | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5f OCA], [http://www.ebi.ac.uk/pdbsum/2a5f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a5f RCSB]</span>
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}}
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'''Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6'''
'''Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6'''
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Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16099990 16099990]
Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16099990 16099990]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: NAD(+)--diphthamide ADP-ribosyltransferase]]
 
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Jobling, M G.]]
[[Category: Jobling, M G.]]
[[Category: Neal, C J.O.]]
[[Category: Neal, C J.O.]]
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[[Category: protein transport/transferase]]
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[[Category: Protein transport/transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:37:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:05 2008''
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Revision as of 15:37, 3 May 2008

Image:2a5f.gif

Template:STRUCTURE 2a5f

Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6


Overview

The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs.

About this Structure

2A5F is a Protein complex structure of sequences from Homo sapiens and Vibrio cholerae. Full crystallographic information is available from OCA.

Reference

Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:16099990 Page seeded by OCA on Sat May 3 18:37:47 2008

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