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2ac1
From Proteopedia
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[[Image:2ac1.gif|left|200px]] | [[Image:2ac1.gif|left|200px]] | ||
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'''Crystal structure of a cell-wall invertase from Arabidopsis thaliana''' | '''Crystal structure of a cell-wall invertase from Arabidopsis thaliana''' | ||
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[[Category: Roy, K Le.]] | [[Category: Roy, K Le.]] | ||
[[Category: Verhaest, M.]] | [[Category: Verhaest, M.]] | ||
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Revision as of 15:51, 3 May 2008
Crystal structure of a cell-wall invertase from Arabidopsis thaliana
Overview
Cell-wall invertases play crucial roles during plant development. They hydrolyse sucrose into its fructose and glucose subunits by cleavage of the alpha1-beta2 glycosidic bond. Here, the structure of the Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1; gene accession code At3g13790) is described at a resolution of 2.15 A. The structure comprises an N-terminal fivefold beta-propeller domain followed by a C-terminal domain formed by two beta-sheets. The active site is positioned in the fivefold beta-propeller domain, containing the nucleophile Asp23 and the acid/base catalyst Glu203 of the double-displacement enzymatic reaction. The function of the C-terminal domain remains unknown. Unlike in other GH 32 family enzyme structures known to date, in AtcwINV1 the cleft formed between both domains is blocked by Asn299-linked carbohydrates. A preliminary site-directed mutagenesis experiment (Asn299Asp) removed the glycosyl chain but did not alter the activity profile of the enzyme.
About this Structure
2AC1 is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
X-ray diffraction structure of a cell-wall invertase from Arabidopsis thaliana., Verhaest M, Lammens W, Le Roy K, De Coninck B, De Ranter CJ, Van Laere A, Van den Ende W, Rabijns A, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1555-63. Epub 2006, Nov 23. PMID:17139091 Page seeded by OCA on Sat May 3 18:51:55 2008
