6bbp
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Model for compact volume of truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L fusion protein== | |
| + | <StructureSection load='6bbp' size='340' side='right' caption='[[6bbp]], [[Resolution|resolution]] 35.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6bbp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BBP FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4IP:INOSITOL-(1,3,4,5)-TETRAKISPHOSPHATE'>4IP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbp OCA], [http://pdbe.org/6bbp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bbp RCSB], [http://www.ebi.ac.uk/pdbsum/6bbp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/CYH3_MOUSE CYH3_MOUSE]] Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP.<ref>PMID:18042453</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Membrane dynamic processes including vesicle biogenesis depend on Arf guanosine triphosphatase (GTPase) activation by guanine nucleotide exchange factors (GEFs) containing a catalytic Sec7 domain and a membrane-targeting module such as a pleckstrin homology (PH) domain. The catalytic output of cytohesin family Arf GEFs is controlled by autoinhibitory interactions that impede accessibility of the exchange site in the Sec7 domain. These restraints can be relieved through activator Arf-GTP binding to an allosteric site comprising the PH domain and proximal autoinhibitory elements (Sec7-PH linker and C-terminal helix). Small-angle X-ray scattering and negative-stain electron microscopy were used to investigate the structural organization and conformational dynamics of cytohesin-3 (Grp1) in autoinhibited and active states. The results support a model in which hinge dynamics in the autoinhibited state expose the activator site for Arf-GTP binding, while subsequent C-terminal helix unlatching and repositioning unleash conformational entropy in the Sec7-PH linker to drive exposure of the exchange site. | ||
| - | + | Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors.,Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG Structure. 2018 Jan 2;26(1):106-117.e6. doi: 10.1016/j.str.2017.11.019. Epub 2017, Dec 21. PMID:29276036<ref>PMID:29276036</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 6bbp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Das, S]] | [[Category: Das, S]] | ||
| - | [[Category: Lambright, D | + | [[Category: Lambright, D G]] |
| - | [[Category: Malaby, A | + | [[Category: Malaby, A W]] |
| + | [[Category: 5-tetrakisphosphate]] | ||
| + | [[Category: Arf gtpase]] | ||
| + | [[Category: Fusion protein]] | ||
| + | [[Category: Guanine nucleotide exchange factor]] | ||
| + | [[Category: Inositol 1]] | ||
| + | [[Category: Lipid binding protein]] | ||
Revision as of 08:31, 10 January 2018
Model for compact volume of truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L fusion protein
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