5y9g
From Proteopedia
(Difference between revisions)
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<StructureSection load='5y9g' size='340' side='right' caption='[[5y9g]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='5y9g' size='340' side='right' caption='[[5y9g]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5y9g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y9G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5y9g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_enteritidis"_gaertner_1888 "bacillus enteritidis" gaertner 1888]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y9G FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y9g OCA], [http://pdbe.org/5y9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y9g RCSB], [http://www.ebi.ac.uk/pdbsum/5y9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y9g ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IN36_14140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=149539 "Bacillus enteritidis" Gaertner 1888])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y9g OCA], [http://pdbe.org/5y9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y9g RCSB], [http://www.ebi.ac.uk/pdbsum/5y9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y9g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pili are critical in host recognition, colonization and biofilm formation during bacterial infection. Here, we report the crystal structures of SafD-dsc and SafD-SafA-SafA (SafDAA-dsc) in Saf pili. Cell adherence assays show that SafD and SafA are both required for host recognition, suggesting a poly-adhesive mechanism for Saf pili. Moreover, the SafDAA-dsc structure, as well as SAXS characterization, reveals an unexpected inter-molecular oligomerization, prompting the investigation of Saf-driven self-association in biofilm formation. The bead/cell aggregation and biofilm formation assays are used to demonstrate the novel function of Saf pili. Structure-based mutants targeting the inter-molecular hydrogen bonds and complementary architecture/surfaces in SafDAA-dsc dimers significantly impaired the Saf self-association activity and biofilm formation. In summary, our results identify two novel functions of Saf pili: the poly-adhesive and self-associating activities. More importantly, Saf-Saf structures and functional characterizations help to define a pili-mediated inter-cellular oligomerizaiton mechanism for bacterial aggregation, colonization and ultimate biofilm formation. | ||
| + | |||
| + | Structural basis of host recognition and biofilm formation by Salmonella Saf pili.,Zeng L, Zhang L, Wang P, Meng G Elife. 2017 Nov 10;6. doi: 10.7554/eLife.28619. PMID:29125121<ref>PMID:29125121</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5y9g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus enteritidis gaertner 1888]] | ||
[[Category: Meng, G]] | [[Category: Meng, G]] | ||
[[Category: Wang, P R]] | [[Category: Wang, P R]] | ||
Revision as of 07:58, 6 December 2017
Crystal structure of Salmonella SafD adhesin
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