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2agv
From Proteopedia
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[[Image:2agv.gif|left|200px]] | [[Image:2agv.gif|left|200px]] | ||
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'''Crystal structure of the SR CA2+-ATPASE with BHQ and TG''' | '''Crystal structure of the SR CA2+-ATPASE with BHQ and TG''' | ||
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[[Category: Obara, K.]] | [[Category: Obara, K.]] | ||
[[Category: Toyoshima, C.]] | [[Category: Toyoshima, C.]] | ||
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| - | [[Category: | + | [[Category: Membrane protein]] |
| - | [[Category: | + | [[Category: P-type atpase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:01:56 2008'' | |
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Revision as of 16:01, 3 May 2008
Crystal structure of the SR CA2+-ATPASE with BHQ and TG
Overview
Ca(2+)-ATPase of sarcoplasmic reticulum is an ATP-powered Ca(2+) pump but also a H(+) pump in the opposite direction with no demonstrated functional role. Here, we report a 2.4-A-resolution crystal structure of the Ca(2+)-ATPase in the absence of Ca(2+) stabilized by two inhibitors, dibutyldihydroxybenzene, which bridges two transmembrane helices, and thapsigargin, also bound in the membrane region. Now visualized are water and several phospholipid molecules, one of which occupies a cleft between two transmembrane helices. Atomic models of the Ca(2+) binding sites with explicit hydrogens derived by continuum electrostatic calculations show how water and protons fill the space and compensate charge imbalance created by Ca(2+)-release. They suggest that H(+) countertransport is a consequence of a requirement for maintaining structural integrity of the empty Ca(2+)-binding sites. For this reason, cation countertransport is probably mandatory for all P-type ATPases and possibly accompanies transport of water as well.
About this Structure
2AGV is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
Structural role of countertransport revealed in Ca(2+) pump crystal structure in the absence of Ca(2+)., Obara K, Miyashita N, Xu C, Toyoshima I, Sugita Y, Inesi G, Toyoshima C, Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14489-96. Epub 2005 Sep 6. PMID:16150713 Page seeded by OCA on Sat May 3 19:01:56 2008
