2air
From Proteopedia
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'''T-state Active Site of Aspartate Transcarbamylase:Crystal Structure of the Carbamyl Phosphate and L-alanosine Ligated Enzyme''' | '''T-state Active Site of Aspartate Transcarbamylase:Crystal Structure of the Carbamyl Phosphate and L-alanosine Ligated Enzyme''' | ||
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[[Category: Huang, J.]] | [[Category: Huang, J.]] | ||
[[Category: Lipscomb, W N.]] | [[Category: Lipscomb, W N.]] | ||
| - | [[Category: | + | [[Category: Alanosine]] |
| - | [[Category: | + | [[Category: Aspartate transcarbamylase]] |
| - | [[Category: | + | [[Category: Carbamyl phosphate]] |
| - | [[Category: | + | [[Category: T-state]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:06:07 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:06, 3 May 2008
T-state Active Site of Aspartate Transcarbamylase:Crystal Structure of the Carbamyl Phosphate and L-alanosine Ligated Enzyme
Overview
An X-ray diffraction study to 2.0 A resolution shows that this enzyme, ATCase, is in the T-state (the c3 to c3 distance is 45.2 A) when ATCase is bound to carbamyl phosphate (CP) and to L-alanosine (an analogue of aspartate). This result strongly supports the kinetic results that alanosine did not inhibit the carbamylation of aspartate in the normal reaction of native ATCase plus CP and aspartate [Baillon, J., Tauc, P., and Herve, G. (1985) Biochemistry 24, 7182-7187]. The structure further reveals that the phosphate of CP is 4 A away from its known position in the R-state and is in the T-state position of P(i) in a recent study of ATCase complexed with products, phosphate (P(i)) and N-carbamyl-L-aspartate [Huang, J., and Lipscomb, W. N. (2004) Biochemistry 43, 6422-6426]. Moreover, the alanosine position in this T-state is somewhat displaced from that expected for its analogue, aspartate, from the R-state position. The relations of these structural aspects to the kinetics are presented.
About this Structure
2AIR is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
T-state active site of aspartate transcarbamylase: crystal structure of the carbamyl phosphate and L-alanosine ligated enzyme., Huang J, Lipscomb WN, Biochemistry. 2006 Jan 17;45(2):346-52. PMID:16401065 Page seeded by OCA on Sat May 3 19:06:07 2008
