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| | ==Crystal structure of N-acetyltransferase from Staphylococcus aureus Mu50== | | ==Crystal structure of N-acetyltransferase from Staphylococcus aureus Mu50== |
| - | <StructureSection load='4lua' size='340' side='right' caption='[[4lua]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='4lua' size='340' side='right'caption='[[4lua]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LUA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lua]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LUA FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mbu|4mbu]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lua OCA], [https://pdbe.org/4lua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lua RCSB], [https://www.ebi.ac.uk/pdbsum/4lua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lua ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAV0826 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lua OCA], [http://pdbe.org/4lua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lua RCSB], [http://www.ebi.ac.uk/pdbsum/4lua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lua ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H3JYD9_STAAM A0A0H3JYD9_STAAM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Staam]] | + | [[Category: Large Structures]] |
| - | [[Category: Forwood, J K]] | + | [[Category: Staphylococcus aureus subsp. aureus Mu50]] |
| - | [[Category: Srivastava, P]] | + | [[Category: Forwood JK]] |
| - | [[Category: Acetyltransferase]] | + | [[Category: Srivastava P]] |
| - | [[Category: Nat_sf]]
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| - | [[Category: Transferase]]
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| Structural highlights
Function
A0A0H3JYD9_STAAM
Publication Abstract from PubMed
Staphylococcus aureus is a prevalent microorganism that is capable of causing a wide range of infections and diseases. Several strains of this bacterial species have developed antibiotic resistance to methicillin and vancomycin, and higher death rates are still being reported each year owing to multidrug-resistant strains. Certain GCN5-related N-acetyltransferases (GNATs) exhibit a broad substrate range, including aminoglycosides, histones, other proteins and serotonin, and have been implicated in antibiotic drug resistance. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis of a GNAT from S. aureus (SaNAT) are reported. SaNAT was recombinantly expressed and crystallized by the hanging-drop vapour-diffusion method at 296 K, and the crystals diffracted to 1.7 A resolution on the MX2 beamline at the Australian Synchrotron. The crystals belonged to space group P43212, with unit-cell parameters a = b = 84.86, c = 49.06 A, alpha = beta = gamma = 90 degrees . A single molecule is likely to be present in the asymmetric unit. A full structural and functional analysis is currently being undertaken to provide novel insights into the protein function, which in turn may provide a basis for drug design.
Purification, crystallization and preliminary X-ray diffraction analysis of the N-acetyltransferase SAV0826 from Staphylococcus aureus.,Srivastava P, Khandokar YB, Forwood JK Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):211-4. doi:, 10.1107/S2053230X13034493. Epub 2014 Jan 21. PMID:24637759[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Srivastava P, Khandokar YB, Forwood JK. Purification, crystallization and preliminary X-ray diffraction analysis of the N-acetyltransferase SAV0826 from Staphylococcus aureus. Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):211-4. doi:, 10.1107/S2053230X13034493. Epub 2014 Jan 21. PMID:24637759 doi:http://dx.doi.org/10.1107/S2053230X13034493
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