2an2

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[[Image:2an2.gif|left|200px]]
[[Image:2an2.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2an2 |SIZE=350|CAPTION= <scene name='initialview01'>2an2</scene>, resolution 2.6&Aring;
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The line below this paragraph, containing "STRUCTURE_2an2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2an2| PDB=2an2 | SCENE= }}
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|RELATEDENTRY=[[1m7z|1M7Z]], [[2ano|2ANO]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2an2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2an2 OCA], [http://www.ebi.ac.uk/pdbsum/2an2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2an2 RCSB]</span>
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}}
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'''P332G, A333S Double mutant of the Bacillus subtilis Nitric Oxide Synthase'''
'''P332G, A333S Double mutant of the Bacillus subtilis Nitric Oxide Synthase'''
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[[Category: Crane, B R.]]
[[Category: Crane, B R.]]
[[Category: Pant, K.]]
[[Category: Pant, K.]]
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[[Category: double mutant]]
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[[Category: Double mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:14:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:54:45 2008''
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Revision as of 16:14, 3 May 2008

Image:2an2.gif

Template:STRUCTURE 2an2

P332G, A333S Double mutant of the Bacillus subtilis Nitric Oxide Synthase


Overview

Cooperativity among ligand binding, subunit association, and protein folding has implications for enzyme regulation as well as protein aggregation events associated with disease. The binding of substrate l-arginine or cofactor tetrahydrobiopterin converts nitric oxide synthases (NOSs) from a "loose dimer", with an exposed active center and higher sensitivity to proteolysis, to a "tight dimer" competent for catalysis. The crystallographic structure of the Bacillus subtilis NOS loose dimer shows an altered association state with severely destabilized subdomains. Ligand binding or heme reduction converts loose dimers to tight dimers in solution and crystals. Mutations at key positions in the dimer interface that distinguish prokaryotic from eukaryotic NOSs affect the propensity to form loose dimers. The loose dimer structure indicates that non-native interactions can mediate subunit association in NOS.

About this Structure

2AN2 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structure of a loose dimer: an intermediate in nitric oxide synthase assembly., Pant K, Crane BR, J Mol Biol. 2005 Sep 30;352(4):932-40. PMID:16126221 Page seeded by OCA on Sat May 3 19:14:24 2008

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OCA

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