2ana
From Proteopedia
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'''THE CRYSTAL STRUCTURE OF D(G-G-G-G-C-C-C-C). A MODEL FOR POLY(DG).POLY(DC)''' | '''THE CRYSTAL STRUCTURE OF D(G-G-G-G-C-C-C-C). A MODEL FOR POLY(DG).POLY(DC)''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANA OCA]. | |
==Reference== | ==Reference== | ||
The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC)., McCall M, Brown T, Kennard O, J Mol Biol. 1985 Jun 5;183(3):385-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/4020865 4020865] | The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC)., McCall M, Brown T, Kennard O, J Mol Biol. 1985 Jun 5;183(3):385-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/4020865 4020865] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Brown, T.]] | [[Category: Brown, T.]] | ||
[[Category: Kennard, O.]] | [[Category: Kennard, O.]] | ||
[[Category: McCall, M.]] | [[Category: McCall, M.]] | ||
| - | [[Category: | + | [[Category: A-dna]] |
| - | [[Category: | + | [[Category: Double helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:14:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:14, 3 May 2008
THE CRYSTAL STRUCTURE OF D(G-G-G-G-C-C-C-C). A MODEL FOR POLY(DG).POLY(DC)
Overview
The structure of the DNA oligomer d(G-G-G-G-C-C-C-C) has been determined at a resolution of 2.5 A by single-crystal X-ray methods. There are two strands in the asymmetric unit, and these coil about each other to form a right-handed double-helix of the A-type with Watson-Crick hydrogen bonds between base-pairs. The helix has a shallow minor groove and a deep, water-filled major groove; almost all exposed functional groups on the DNA are hydrated, and 106 ordered solvent molecules have been found. The two d(G-G-G-G).d(C-C-C-C) segments in the octamer exhibit similar and uniform structures, but there is a slight discontinuity at the GpC step between them. A recurring feature of the structure is the overlap of adjacent guanine bases in each GpG step, with the five-membered ring of one guanine stacking on the six-membered ring of its neighbour. There is little or no overlap between adjacent cytosine rings. Conformational parameters for these GpG steps are compared with those from other single-crystal X-ray analyses. In general, GpG steps exhibit high slide, low roll and variable twist. Models for poly(dG).poly(dC) were generated by applying a simple rotation and translation to each of the unmodified d(G-G-G-G).d(C-C-C-C) units. Detailed features of these models are shown to be compatible with various assays of poly(dG).poly(dC) in solution, and are useful in understanding the polymorphic behaviour of this sequence under a variety of experimental conditions.
About this Structure
Full crystallographic information is available from OCA.
Reference
The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC)., McCall M, Brown T, Kennard O, J Mol Biol. 1985 Jun 5;183(3):385-96. PMID:4020865 Page seeded by OCA on Sat May 3 19:14:52 2008
