This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4n8v

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 08:15, 11 January 2023

Crystal structure of killer cell immunoglobulin-like receptor KIR2DS2 in complex with HLA-A

Structural highlights

4n8v is a 8 chain structure with sequence from Homo sapiens and Vaccinia virus WR. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KI2S2_HUMAN Receptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells.

Publication Abstract from PubMed

Inhibitory killer cell Ig-like receptors (KIRs) are known to recognize HLA ligands mainly of the HLA-C and Bw4 groups, but the ligands for KIRs are poorly understood. We report here the identification of the cognate ligand for the activating KIR 2DS2 as HLA-A*11:01. The crystal structure of the KIR2DS2-HLA-A*11:01 complex was solved at 2.5-A resolution and revealed residue-binding characteristics distinct from those of inhibitory KIRs with HLA-C and the critical role of residues Tyr45 and Asp72 in shaping binding specificity to HLA-A*11:01. Using KIR2DS2 tetramers, binding to surface HLA-A*11:01 on live cells was demonstrated and, furthermore, that binding can be altered by residue changes at p8 of the peptide, indicating the influence of peptide sequence on KIR-HLA association. In addition, heteronuclear single quantum coherence NMR was used to map the involvement of critical residues in HLA binding at the interface of KIR and HLA, and validates the data observed in the crystal structure. Our data provide structural evidence of the recognition of A*11:01 by the activating KIR2DS2 and extend our understanding of the KIR-HLA binding spectrum.

Activating killer cell immunoglobulin-like receptor 2DS2 binds to HLA-A*11.,Liu J, Xiao Z, Ko HL, Shen M, Ren EC Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2662-7. doi:, 10.1073/pnas.1322052111. Epub 2014 Feb 3. PMID:24550293^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu J, Xiao Z, Ko HL, Shen M, Ren EC. Activating killer cell immunoglobulin-like receptor 2DS2 binds to HLA-A*11. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2662-7. doi:, 10.1073/pnas.1322052111. Epub 2014 Feb 3. PMID:24550293 doi:http://dx.doi.org/10.1073/pnas.1322052111

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4n8v"

Categories: Homo sapiens | Large Structures | Vaccinia virus WR | Liu JX | Ren EC

@@ Line 1: / Line 1: @@
 ==Crystal structure of killer cell immunoglobulin-like receptor KIR2DS2 in complex with HLA-A==
-<StructureSection load='4n8v' size='340' side='right' caption='[[4n8v]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='4n8v' size='340' side='right'caption='[[4n8v]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4n8v]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N8V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4n8v]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N8V FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KIR2DS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), HLA-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), B2M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n8v OCA], [https://pdbe.org/4n8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n8v RCSB], [https://www.ebi.ac.uk/pdbsum/4n8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n8v ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n8v OCA], [http://pdbe.org/4n8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n8v RCSB], [http://www.ebi.ac.uk/pdbsum/4n8v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n8v ProSAT]</span></td></tr>
 </table>
-== Disease ==
-[[http://www.uniprot.org/uniprot/B2MG_HUMAN B2MG_HUMAN]] Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:[http://omim.org/entry/241600 241600]]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.<ref>PMID:16549777</ref>   Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.<ref>PMID:3532124</ref> <ref>PMID:1336137</ref> <ref>PMID:7554280</ref> <ref>PMID:4586824</ref> <ref>PMID:8084451</ref> <ref>PMID:12119416</ref> <ref>PMID:12796775</ref> <ref>PMID:16901902</ref> <ref>PMID:16491088</ref> <ref>PMID:17646174</ref> <ref>PMID:18835253</ref> <ref>PMID:18395224</ref> <ref>PMID:19284997</ref>
 == Function ==
-[[http://www.uniprot.org/uniprot/A14_VACCW A14_VACCW]] Envelope protein which is a major component of the mature virion (MV) membrane. Essential for membrane biogenesis. Is required, together with A17, to form bona fide crescents, which can progress to form the immature virion (IV) membrane. A14 and A17 form a lattice that is stabilized by disulfide bonds and serves as an anchor within the viral membrane to which several other proteins important in virion structure and morphogenesis attach.<ref>PMID:9445029</ref> <ref>PMID:10729144</ref>  [[http://www.uniprot.org/uniprot/KI2S2_HUMAN KI2S2_HUMAN]] Receptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells. [[http://www.uniprot.org/uniprot/B2MG_HUMAN B2MG_HUMAN]] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [[http://www.uniprot.org/uniprot/1A11_HUMAN 1A11_HUMAN]] Involved in the presentation of foreign antigens to the immune system.
+[https://www.uniprot.org/uniprot/KI2S2_HUMAN KI2S2_HUMAN] Receptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 17: @@
 </div>
 <div class="pdbe-citations 4n8v" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
+*[[NK cell receptor|NK cell receptor]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Liu, J X]]
+[[Category: Large Structures]]
-[[Category: Ren, E C]]
+[[Category: Vaccinia virus WR]]
-[[Category: Activating kir]]
+[[Category: Liu JX]]
-[[Category: Antigen presentation]]
+[[Category: Ren EC]]
-[[Category: Cell lysing]]
-[[Category: Immune system]]
-[[Category: Kir binding]]
-[[Category: Kir2ds2]]
-[[Category: Membrane]]

4n8v

From Proteopedia

Revision as of 08:15, 11 January 2023

Crystal structure of killer cell immunoglobulin-like receptor KIR2DS2 in complex with HLA-A

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox